Marking through molts: An evaluation of visible implant elastomer to permanently mark individuals in a lower termite species.
Marking through molts: An evaluation of visible implant elastomer to permanently mark individuals in a lower termite species.
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通过蜕皮标记：对可见植入弹性体永久标记低等白蚁物种个体的评估。
DOI：
10.1002/ece3.8030
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发表时间：
2021-09
影响因子：
2.6
通讯作者：
Thompson FJ
中科院分区：
文献类型：
--
作者：
Padget RFB;Thompson FJ
个体标记方法的进步促进了对动物种群和行为的详细研究，因为它们允许通过时间和空间跟踪个体。半变态昆虫代表了广泛使用的模式生物，对个体标记提出了独特的挑战，因为它们不仅通常身体较小，而且在整个发育过程中都会蜕皮，这意味着传统的表面标记不是持久的。可见植入弹性体(VIE)提供了一种潜在的解决方案，因为少量的惰性聚合物可以植入动物的皮肤或角质层下。VIE已被证明在野外和实验室研究中对单独标记鱼、甲壳类和两栖动物很有用，最近在实验室蠕虫和苍蝇幼虫种群中成功地进行了试验。我们在单件筑巢的白蚁Zooteropsis angusticollis上进行了VIE试验，这是一种小型半变态昆虫。我们发现，VIE对存活没有影响，而且在蜕皮后仍有痕迹。然而，我们发现一些证据表明，有标记的白蚁比对照白蚁表现出更少的同种异体修饰和滋养作用，尽管影响很小。我们的研究表明，VIE是标记白蚁等小型半变态昆虫的有效技术，但我们主张谨慎应用，特别是在行为观察很重要的情况下。标记个体可以对行为和生活史进行详细研究，但对经历蜕皮的物种永久标记个体是出了名的困难。在这里，我们使用可见植入弹性体(VIE)来标记白蚁的个体。我们表明，使用VIE标记对生存或行为几乎没有影响，重要的是，VIE标记在蜕皮过程中持续存在。
Advances in individual marking methods have facilitated detailed studies of animal populations and behavior as they allow tracking of individuals through time and space. Hemimetabolous insects, representing a wide range of commonly used model organisms, present a unique challenge to individual marking as they are not only generally small‐bodied, but also molt throughout development, meaning that traditional surface marks are not persistent. Visible implant elastomer (VIE) offers a potential solution as small amounts of the inert polymer can be implanted under the skin or cuticle of an animal. VIE has proved useful for individually marking fish, crustaceans, and amphibians in both field and laboratory studies and has recently been successfully trialed in laboratory populations of worms and fly larvae. We trialed VIE in the single‐piece nesting termite Zootermopsis angusticollis, a small hemimetabolous insect. We found that there was no effect of VIE on survival and that marks persisted following molting. However, we found some evidence that marked termites performed less allogrooming and trophallaxis than controls, although effect sizes were very small. Our study suggests that VIE is an effective technique for marking small hemimetabolous insects like termites but we advocate that caution is applied, particularly when behavioral observation is important. Marking individuals allows detailed studies of behavior and life history, but permanently marking individuals in species that undergo molts is notoriously difficult. Here we use visible implant elastomer (VIE) to mark individuals in a species of termite, Zootermopsis angusticollis. We show that marking using VIE has little effect on survival or behavior and, importantly, VIE marks persist through molts.
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影响因子：
2.5
作者：
Hoffmann, Katharina;Korb, Judith
通讯作者：
Korb, Judith
DOI：
10.1073/pnas.0907961106
发表时间：
2009-10-13
影响因子：
11.1
作者：
Johns, Philip M.;Howard, Kenneth J.;Thorne, Barbara L.
通讯作者：
Thorne, Barbara L.
影响因子：
2.3
作者：
Korb, J;Schmidinger, S
通讯作者：
Schmidinger, S
影响因子：
4.8
作者：
Butt, Kevin R.;Lowe, Christopher N.
通讯作者：
Lowe, Christopher N.
影响因子：
3.7
作者：
Freelance, Christopher B.
通讯作者：
Freelance, Christopher B.