Identification of multiple potential viral diseases in a large urban center using wastewater surveillance
Identification of multiple potential viral diseases in a large urban center using wastewater surveillance
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DOI：
10.1016/j.watres.2020.116160
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发表时间：
2020-10-01
期刊：
影响因子：
12.8
通讯作者：
Xagoraraki, Irene
中科院分区：
文献类型：
--
作者：
McCall, Camille;Wu, Huiyun;Xagoraraki, Irene
病毒与多种人类疾病有关，并可在城市化环境中广泛传播，对社区和医疗保健基础设施造成全球不利影响。采用基于废水的流行病学方法，使用元基因组学和定量聚合酶链式反应(QPCR)分析，鉴定从大城市地区收集的肠道和非肠道病毒，以进行潜在的公共卫生监测和暴发分析。从2017年11月至2018年2月收集未经处理的废水样本(n 1/4 54)，以评估收集样本中人类病毒病原体的多样性。根据主要传播途径将病毒分为病毒类型，并针对集水区报告的病毒相关疾病进行审查。元基因组学检测到在抽样年期间研究区域内报告的引起临床重大疾病的病毒病原体的存在。检测到的病毒属于腺病毒科、天星病毒科、杯状病毒科、冠状病毒科、黄病毒科、海佩病毒科、疱疹病毒科、马托纳病毒科、乳头病毒科、细小病毒科、短小病毒科、痘病毒科、逆转录病毒科和豆科病毒科。此外，还检测了废水样本中的腺病毒、诺如病毒GII、SAP病毒、甲型肝炎病毒、人类疱疹病毒6和人类疱疹病毒8的浓度，并与后基因组学结果进行比较，以确认检测到的病毒属。甲型肝炎病毒在废水样本中的平均病毒载量最高(1.86×10(7)基因组拷贝/L)，与其他病毒相比，在基因组样本中的检测率为100%。萨普病毒的平均浓度(1.36×10(6)个基因组拷贝/L)显着高于诺如病毒GII(2.94×10(4)个基因组拷贝/L)，表明研究区域内的负担较高。这项研究的结果有助于评估基于废水的流行病学在大型社区识别和常规监测各种病毒方面的效用。这种方法有可能改善公共卫生对大规模暴发和病毒大流行的反应。(C)2020爱思唯尔有限公司。保留所有权利。
Viruses are linked to a multitude of human illnesses and can disseminate widely in urbanized environments causing global adverse impacts on communities and healthcare infrastructures. Wastewater-based epidemiology was employed using metagenomics and quantitative polymerase chain reaction (qPCR) assays to identify enteric and non-enteric viruses collected from a large urban area for potential public health monitoring and outbreak analysis. Untreated wastewater samples were collected from November 2017 to February 2018 (n 1/4 54) to evaluate the diversity of human viral pathogens in collected samples. Viruses were classified into virus types based on primary transmission routes and reviewed against viral associated diseases reported in the catchment area. Metagenomics detected the presence of viral pathogens that cause clinically significant diseases reported within the study area during the sampling year. Detected viruses belong to the Adenoviridae, Astroviridae, Caliciviridae, Coronaviridae, Flaviviridae, Hepeviridae, Herpesviridae, Matonaviridae, Papillomaviridae, Parvoviridae, Picornaviridae, Poxviridae, Retroviridae, and Togaviridae families. Furthermore, concentrations of adenovirus, norovirus GII, sapovirus, hepatitis A virus, human herpesvirus 6, and human herpesvirus 8 were measured in wastewater samples and compared to metagenomic findings to confirm detected viral genus. Hepatitis A virus obtained the greatest average viral load (1.86 x 10(7) genome copies/L) in wastewater samples compared to other viruses quantified using qPCR with a 100% detection rate in metagenomic samples. Average concentration of sapovirus (1.36 x 10(6) genome copies/L) was significantly greater than norovirus GII (2.94 x 10(4) genome copies/L) indicating a higher burden within the study area. Findings obtained from this study aid in evaluating the utility of wastewater-based epidemiology for identification and routine monitoring of various viruses in large communities. This methodology has the potential to improve public health responses to large scale outbreaks and viral pandemics. (c) 2020 Elsevier Ltd. All rights reserved.