ATAD2 expression increases [18F]Fluorodeoxyglucose uptake value in lung adenocarcinoma via AKT-GLUT1/HK2 pathway
ATAD2 expression increases [18F]Fluorodeoxyglucose uptake value in lung adenocarcinoma via AKT-GLUT1/HK2 pathway
复制标题
ATAD2 表达通过 AKT-GLUT1/HK2 途径增加肺腺癌中的[18F]氟脱氧葡萄糖摄取值
DOI：
10.5483/bmbrep.2019.52.7.042
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发表时间：
2019-07-31
期刊：
影响因子：
3.8
通讯作者：
Li, Yaming
中科院分区：
文献类型：
--
作者：
Sun, Tong;Du, Bulin;Li, Yaming
[18 F]氟脱氧葡萄糖（FDG）PET/CT显像已广泛应用于恶性肿瘤的诊断。ATP酶家族AAA结构域蛋白2（ATAD 2）在肿瘤的生长、侵袭和转移中起重要作用。然而，[18 F]FDG蓄积与ATAD 2表达之间的关系在很大程度上仍然未知。本研究旨在探讨肺腺癌（LUAD）中ATAD 2表达与[18 F]FDG摄取的相关性，并阐明其分子机制。结果显示，LUAD组织中ATAD 2的表达与最大标准摄取值（SUVmax）、总病变糖酵解（TLG）、葡萄糖转运蛋白1（GLUT 1）和己糖激酶2（HK 2）的表达呈正相关。ATAD 2基因敲减可显著抑制LUAD细胞的增殖、致瘤性、迁移、[18 F]FDG摄取和乳酸产生，而ATAD 2基因过表达则表现出相反的作用。此外，ATAD 2通过AKT-GLUT 1/HK 2途径调节LUAD的糖代谢，如使用LY 294002（PI 3 K/AKT途径的抑制剂）评估的。因此，探讨ATAD 2表达与糖代谢的相关性，有望为肿瘤的抗能量代谢治疗带来新的希望。
[18F]Fluorodeoxyglucose (FDG) PET/CT imaging has been widely used in the diagnosis of malignant tumors. ATPase family AAA domain-containing protein 2 (ATAD2) plays important roles in tumor growth, invasion and metastasis. However, the relationship between [18F]FDG accumulation and ATAD2 expression remains largely unknown. This study aimed to investigate the correlation between ATAD2 expression and [18F]FDG uptake in lung adenocarcinoma (LUAD), and elucidate its underlying molecular mechanisms. The results showed that ATAD2 expression was positively correlated with maximum standardized uptake value (SUVmax), total lesion glycolysis (TLG), glucose transporter type 1 (GLUT1) expression and hexokinase2 (HK2) expression in LUAD tissues. In addition, ATAD2 knockdown significantly inhibited the proliferation, tumorigenicity, migration, [18F]FDG uptake and lactate production of LUAD cells, while, ATAD2 overexpression exhibited the opposite effects. Furthermore, ATAD2 modulated the glycometabolism of LUAD via AKT-GLUT1/HK2 pathway, as assessed using LY294002 (an inhibitor of PI3K/AKT pathway). In summary, to explore the correlation between ATAD2 expression and glycometabolism is expected to bring good news for anti-energy metabolism therapy of cancers.