K562 HUMAN-LEUKEMIC CELLS SYNTHESIZE EMBRYONIC HEMOGLOBIN IN RESPONSE TO HEMIN
K562 HUMAN-LEUKEMIC CELLS SYNTHESIZE EMBRYONIC HEMOGLOBIN IN RESPONSE TO HEMIN
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DOI：
10.1038/280164a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
WEATHERALL, DJ
中科院分区：
文献类型：
--
作者：
RUTHERFORD, TR;CLEGG, JB;WEATHERALL, DJ
由于缺乏可检测红细胞分化的自我维持培养物，以及难以获得胚胎和早期胎儿的红细胞生成组织，阻碍了对不同发育阶段人类红细胞生成和血红蛋白合成调控的研究1。通过研究可在体外诱导分化的红白血病细胞（Friend细胞）培养物，获得了对小鼠红细胞生成调控的新见解2。最近，Andersson等3，4报道了从慢性髓性白血病（CML）急性转化患者中获得的K562人细胞系具有与红系细胞相同的特性。特别是，这种细胞系的细胞膜中含有血型糖蛋白和血影蛋白，并合成微量的血红蛋白，可通过放射免疫测定法检测。我们研究了这些细胞在体外分化的能力，并在这里报告说，它们是由氯化血红素诱导合成大量血红蛋白的;初步分析表明，这主要是胚胎型的。
STUDIES of the regulation of human erythropoiesis and haemoglobin synthesis at different phases of development are hampered by the lack of a self-sustaining culture in which erythroid differentiation can be examined, and by difficulties in obtaining erythropoietic tissue in embryonic and early fetal life1. New insights into the regulation of murine erythropoiesis have been obtained by the study of established cultures of erythro-leukaemic cells (Friend cells) which can be induced to differentiatein vitro2. Recently, Anderssonet al.3,4have reported that the K562 human cell line, obtained from a patient with an acute transformation of chronic myeloid leukaemia (CML) has properties in common with erythroid cells. In particular, cells of this line contain glycophorin and spectrin in their membranes and synthesise minute amounts of haemoglobin, detectable by radioimmunoassay. We have investigated the capacity of these cells to differentiatein vitroand report here that they are induced by haemin to synthesise large amounts of haemoglobin; preliminary analysis indicates that this is predominantly of the embryonic type.