AN IN-VITRO ASSAY UTILIZING PARASITIC LARVAL HAEMONCHUS-CONTORTUS TO DETECT RESISTANCE TO CLOSANTEL AND OTHER ANTHELMINTICS
AN IN-VITRO ASSAY UTILIZING PARASITIC LARVAL HAEMONCHUS-CONTORTUS TO DETECT RESISTANCE TO CLOSANTEL AND OTHER ANTHELMINTICS
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DOI：
10.1016/0020-7519(93)90162-r
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发表时间：
1993-08-01
影响因子：
4
通讯作者：
SANGSTER, NC
中科院分区：
文献类型：
--
作者：
ROTHWELL, JT;SANGSTER, NC
描述了一种体外检测驱虫药耐药性的新方法。该测定使用简单的培养系统，其中评估驱虫药杀死或抑制寄生第三阶段和第四阶段捻转血矛线虫幼虫通过50μm孔径网迁移的能力。该测定检测出 2-10 倍的氯氰碘灵耐药性。还可以检测到对苯并咪唑、左旋咪唑和伊维菌素的耐药性。
A new assay for detecting resistance to anthelmintics in vitro is described. The' assay uses a simple culture system in which the ability of anthelmintics to kill or inhibit the migration of parasitic third and fourth stage Haemonchus contortus larvae through a 50 pm aperture mesh is assessed. The assay detects 2-10-fold resistance to closantel. Resistance to benzimidazoles, levamisole and ivermectin can also be detected.