Cytokines and growth factors in the protruded intervertebral disc of the lumbar spine
Cytokines and growth factors in the protruded intervertebral disc of the lumbar spine
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DOI：
10.1007/s00586-001-0361-y
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发表时间：
2002-04-01
影响因子：
2.8
通讯作者：
Greco, F
中科院分区：
文献类型：
--
作者：
Specchia, N;Pagnotta, A;Greco, F
椎间盘产生的因素引起的神经根刺激可能在坐骨神经痛产生的病理生理学中起着至关重要的作用。在这项研究中，我们使用免疫组织化学方法研究了 30 例椎间盘突出症（突出型）患者的腰椎间盘活检标本中转化生长因子-β1（TGF-β1）、胰岛素样生长因子-1（IGF-1）、白介素-6（IL-6）、IL-6-受体（IL-6R）和纤连蛋白的存在。椎间盘突出的软骨细胞 TGF-β1、IGF-1、IL-6 和纤连蛋白染色呈阳性。我们首次证明了突出组织的软骨细胞中存在 IL-6-R。来自自体健康组织的样本用作对照。在这些切片中，没有发现 TGF-β1、IL-6 或 IL-6R 的免疫反应，而它们表达 IGF-1 和纤连蛋白，但含量低于椎间盘突出。这些结果表明腰椎间盘突出部位产生TGF-β1、IGF-1、IL-6、IL-6R和纤连蛋白等因子。
Nerve root irritation induced by factors produced by the intervertebral disc may play a crucial role in the pathophysiology of sciatic pain production. In this study we used immunohistochemistry to investigate the presence of transforming growth factor-beta1 (TGF-beta1), insulin-like growth factor-1 (IGF-1), interleukin-6 (IL-6), IL-6-receptor (IL-6R) and fibronectin in lumbar disc bioptic specimens from 30 patients with disc herniation (protrusion type). Chondrocytes of herniated discs stained positive for TGF-beta1, IGF-1, IL-6 and fibronectin. We demonstrated for the first time the presence of IL-6-R in the chondrocytes of herniated tissue. Specimens from autoptic healthy tissue were used as controls. In these sections no immunoreaction for TGF-beta1, IL-6, or IL-6R was found, while they expressed IGF-1 and fibronectin, but in lower quantities than herniated discs. These results demonstrated the production of factors such as TGF-beta1, IGF-1, IL-6, IL-6R and fibronectin at the site of lumbar disc herniation.