Circulating Cell-Free DNA in Dogs with Mammary Tumors: Short and Long Fragments and Integrity Index.
Circulating Cell-Free DNA in Dogs with Mammary Tumors: Short and Long Fragments and Integrity Index.
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DOI：
10.1371/journal.pone.0169454
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Zappulli V
中科院分区：
文献类型：
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作者：
Beffagna G;Sammarco A;Bedin C;Romualdi C;Mainenti M;Mollo A;Cavicchioli L;Ferro S;Trez D;De Maria R;Nitti D;Saccani A;Campanella M;Agostini M;Zappulli V
循环无细胞DNA（cfDNA）已被认为是荷瘤受试者中令人感兴趣的诊断/预后血浆生物标志物。在癌症患者中，cfDNA可以假设来源于肿瘤坏死/凋亡、溶解的循环细胞和一些尚未揭示的活性释放机制。本研究旨在初步分析患有犬乳腺肿瘤（CMT）的犬的cfDNA。招募了44只肿瘤性犬、17只非肿瘤性疾病犬和15只健康犬。还将坏死和凋亡评估为从44只狗诊断的78个CMT上cfDNA的潜在来源。cfDNA片段和完整性指数显著区分肿瘤性与非肿瘤性狗（P<0.05），并且允许区分良性和恶性病变（P<0.05）。即使没有统计学显著性，cfDNA的量也受到肿瘤坏死的影响，并与肿瘤大小和凋亡标志物表达相关。Bcl-2在恶性肿瘤中表达明显增高（P<0.01），转移性CMT中Bcl-2的表达也有逃避凋亡的现象。因此，本研究提供了cfDNA可能是携带乳腺结节的犬的诊断标志物的证据，表明其在早期诊断程序中的潜在应用应进一步研究。
Circulating cell-free DNA (cfDNA) has been considered an interesting diagnostic/prognostic plasma biomarker in tumor-bearing subjects. In cancer patients, cfDNA can hypothetically derive from tumor necrosis/apoptosis, lysed circulating cells, and some yet unrevealed mechanisms of active release. This study aimed to preliminarily analyze cfDNA in dogs with canine mammary tumors (CMTs). Forty-four neoplastic, 17 non-neoplastic disease-bearing, and 15 healthy dogs were recruited. Necrosis and apoptosis were also assessed as potential source of cfDNA on 78 CMTs diagnosed from the 44 dogs. The cfDNA fragments and integrity index significantly differentiated neoplastic versus non-neoplastic dogs (P<0.05), and allowed the distinction between benign and malignant lesions (P<0.05). Even if without statistical significance, the amount of cfDNA was also affected by tumor necrosis and correlated with tumor size and apoptotic markers expression. A significant (P<0.01) increase of Bcl-2 in malignant tumors was observed, and in metastatic CMTs the evasion of apoptosis was also suggested. This study, therefore, provides evidence that cfDNA could be a diagnostic marker in dogs carrying mammary nodules suggesting that its potential application in early diagnostic procedures should be further investigated.