A network-based method for mechanistic investigation of Shexiang Baoxin Pill's treatment of cardiovascular diseases.
A network-based method for mechanistic investigation of Shexiang Baoxin Pill's treatment of cardiovascular diseases.
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基于网络的麝香保心丸治疗心血管疾病机制研究
DOI：
10.1038/srep43632
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发表时间：
2017-03-08
影响因子：
4.6
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Fang HY;Zeng HW;Lin LM;Chen X;Shen XN;Fu P;Lv C;Liu Q;Liu RH;Zhang WD;Zhao J
麝香保心丸是我国治疗心血管疾病的常用中药。然而，其组成和靶点的复杂性阻碍了我们对其作用机制的理解。利用基于网络药理学的方法，我们通过分析蛋白质-蛋白质相互作用和途径，建立了SBP治疗CVD的作用机制。在基于微阵列的研究中，在基因表达水平上证实了计算结果。其中两个SBP的靶点通过Western blot在蛋白质水平上得到进一步证实。此外，我们还验证了SBP血浆吸收物在治疗CVD中起主要治疗作用的理论。
Shexiang Baoxin Pill (SBP), a traditional Chinese medicine formula, is commonly used to treat cardiovascular disease (CVD) in China. However, the complexity of composition and targets has deterred our understanding of its mechanism of action. Using network pharmacology-based approaches, we established the mechanism of action for SBP to treat CVD by analyzing protein-protein interactions and pathways. The computational results were confirmed at the gene expression level in microarray-based studies. Two of the SBP’s targets were further confirmed at the protein level by Western blot. In addition, we validated the theory that SBP’s plasma absorbed compounds play major therapeutic role in treating CVD.