High frequency early flowering from in vitro seedlings of Dendrobium nobile
High frequency early flowering from in vitro seedlings of Dendrobium nobile
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DOI：
10.1016/j.scienta.2009.05.018
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发表时间：
2009-09
影响因子：
4.3
通讯作者：
Z. Wang;L. Wang;Q. Ye
中科院分区：
文献类型：
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作者：
Z. Wang;L. Wang;Q. Ye
石斛是一种受欢迎的温带中国兰花，通常作为传统药用植物销售。金钗石斛幼苗。在含有0.5mgL-1多效唑（PP 333）或0.1mgL-1噻苯隆（TDZ）的1/2 MS培养基上培养4个月，可早熟产生花芽（33.3-34.8%）。在含有PN（PP_（333）0.3mgL-1+NAA 0.5mgL-1）的培养基上生长，再转移到含有PP_（333）和TDZ（PP_（333）+TDZ）的1/2 MS培养基上，花芽形成率可进一步提高到95.6%。然而，在25°C下发育的花是变形的，但当在较低温度制度（23°C/18°C，光/暗）下生长45天时，它完全发育。在最佳条件下，播种后6个月左右观察到试管开花。
Dendrobium nobile Lindl. is a popular temperate Chinese orchid commonly marketed as a traditional medicinal plant. Seedlings of Dendrobium nobile Lindl. produced floral buds (33.3–34.8%) precociously on a defined basal medium (1/2 MS) containing paclobutrazol (PP333) at 0.5mgL−1or thidiazuron (TDZ) at 0.1mgL−1within 4 months of culturing. The frequency of floral buds formation can be further increased to 95.6% by growing seedlings in a PN (PP3330.3mgL−1+NAA 0.5mgL−1)-containing medium followed by transfer onto 1/2 MS medium with PP333and TDZ (PP333+TDZ). However, flower developed was deformed under 25°C but it developed fully when grown in a lower temperature regime (23°C/18°C, light/dark) for 45 days. Under optimal condition, in vitro flowering was observed about 6 months after seed sowing.