Reduced plasma antioxidant concentrations and increased oxidative DNA damage in inflammatory bowel disease
Reduced plasma antioxidant concentrations and increased oxidative DNA damage in inflammatory bowel disease
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DOI：
10.1080/003655201317097146
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发表时间：
2001-12-01
影响因子：
1.9
通讯作者：
Sturniolo, GC
中科院分区：
文献类型：
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作者：
D'Odorico, A;Bortolan, S;Sturniolo, GC
背景：氧化应激被认为在炎症性肠病(IBD)相关性肠损伤的发病机制中起关键作用。循环中的抗氧化剂可能在防止自由基介导的组织损伤方面发挥作用。方法：测定46例溃疡性结肠炎(UC)、37例克罗恩病(CD)患者和386例正常对照的血浆维生素A、维生素E和类胡萝卜素浓度、白细胞基因组损伤和8-羟基脱氧鸟苷(8-OHdG)浓度。从每个受试者身上取20毫升血液样本进行抗氧化剂和8-OHdG测定。对每组受试者的样本进行食物频率问卷调查，以评估每日饮食化合物的摄入量。结果：与对照组相比，炎症性肠病患者的抗氧化剂浓度显著降低，尤其是活动性疾病患者(P<；0.0001)。IBD患者8-OHdG浓度显著高于对照组，与疾病活动无关(P<；0.05)。抗氧化剂和8-OHdG浓度之间没有相关性。与体重指数正常或偏高的IBD患者相比，营养不良的IBD患者的类胡萝卜素浓度(0.89+/-0.14mU/L)显著降低(P<；0.05)，与疾病的活动性或延展性无关。IBD患者蛋白质、水果和蔬菜的摄入量明显低于对照组。结论：抗氧化剂的耗竭可能在IBD的病理生理学中起重要作用：UC和CD患者表现为自由基外周血白细胞DNA损伤增加，血浆抗氧化防御能力降低。这些结果表明有必要进行进一步的研究，以确定最佳维生素状态是否可以改善UC和CD的临床病程。
Background: Oxidative stress is believed to play a key role in the pathogenesis of inflammatory bowel disease (IBD)-related intestinal damage. Circulating antioxidants may have a role to play in preventing free radical-mediated tissue injury. Methods: Plasma vita-min A, E and carotenoid concentrations, leukocytic genomic damage and 8-hydroxy-deoxy-guanosine (8-OHdG) concentration were determined in 46 ulcerative colitis (UC) patients, 37 Crohn disease (CD) patients and 386 controls. A 20 ml blood sample was taken from each subject for antioxidant and 8-OHdG measurements. A food frequency questionnaire was administered to a sample of subjects from each group to evaluate daily intake of dietary compounds. Results: Antioxidant concentration was significantly reduced in IBD patients, particularly in those with active disease, with respect to controls (P < 0.0001). 8-OHdG concentrations were significantly increased in IBD patients compared to controls, independent of disease activity (P < 0.05). No correlation was found between antioxidant and 8-OHdG concentrations. Carotenoid concentrations were significantly reduced in malnourished IBD patients (0.89 +/- 0.14 mu mol/l) compared to patients with normal or high body mass index (1.83 +/- 0.12 mu mol/l; P < 0.05), independent of disease activity or extension. Protein, fruit and vegetable intakes of IBD patients were significantly lower than those of controls. Conclusions: Depletion of antioxidants is likely to be important in the pathophysiology of IBD: UC and CD patients show increased free radical peripheral leukocyte DNA damage and decreased plasma antioxidant defenses. These results indicate the necessity of further studies to establish whether optimal vitamin status may improve the clinical course of UC and CD.