Construction of zinc-incorporated nano-network structures on a biomedical titanium surface to enhance bioactivity
Construction of zinc-incorporated nano-network structures on a biomedical titanium surface to enhance bioactivity
复制标题
在生物医用钛表面构建掺锌纳米网络结构以增强生物活性
DOI：
10.1016/j.apsusc.2018.05.097
复制
发表时间：
2018
影响因子：
6.7
通讯作者：
cai Kaiyong
中科院分区：
文献类型：
--
作者：
Deng Conghui;Shen Xinkun;Yang Weihu;Luo Zhong;Ma Pingping;Shen Tingting;Liu Ju;cai Kaiyong
表面形貌被认为是影响骨整合的关键特性;因此，形貌修饰是钛基种植体研究中最常用的技术。在这项研究中，乙基纤维素作为添加剂，构建一个锌纳入纳米网络层到钛表面的顺序处理的旋涂，高温煅烧，碱热腐蚀。SEM结果表明，当乙基纤维素的质量浓度为20 mg/mL时，涂层表面较为平整，腐蚀时间仅为4 h，涂层表面形成了理想的纳米网络结构。XPS和ICP的其他结果进一步证明了锌离子成功地掺入到最终样品（Ti-Zn 0.1、Ti-Zn 0.3和Ti-Zn 0.4）中。此外，体外细胞（例如，CCK-8、ALP、矿化）和细菌实验表明，Ti-Zn 0.3基质不仅对成骨细胞具有最强的增殖和分化能力，而且对大肠杆菌和金黄色葡萄球菌都具有较强的抗菌能力。本研究为在钛表面快速构建具有促骨生成和抗菌作用的纳米网络结构提供了新的途径。
Surface topography had been identified as a crucial property that affects osseointegration; thus, topographical modification was the most frequently adopted technique in titanium-based implant research. In this study, ethyl cellulose was employed as an additive to construct a zinc-incorporated nano-network layer onto a titanium surface by the sequential treatments of spin-coating, high-temperature calcination, and alkali heat corrosion. SEM results showed that 20 mg/mL of ethyl cellulose was optimal to fabricate a relatively flat porous coating, and the ideal nano-network structures formed by only 4 h of corrosion. Other results of XPS and ICP further proved that zinc ions were successfully incorporated into the final samples (Ti-Zn0.1, Ti-Zn0.3, and Ti-Zn0.4). Moreover, thein vitrocellular (e.g., CCK-8, ALP, mineralization) and bacterial assays presented that Ti-Zn0.3 substrates not only had the greatest proliferation and differentiation capacities for osteoblasts but also possessed relatively strong antibacterial abilities for bothEscherichia coliandStaphylococcus aureus. This study provided a new way to rapidly construct the pro-osteogenesis and antibacterial nano-network structures on titanium surfaces for orthopedic application.