Histone methyltransferase G9a is a key regulator of the starvation-induced behaviors in Drosophila melanogaster.
Histone methyltransferase G9a is a key regulator of the starvation-induced behaviors in Drosophila melanogaster.
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DOI：
10.1038/s41598-017-15344-2
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发表时间：
2017-11-07
影响因子：
4.6
通讯作者：
Yamaguchi M
中科院分区：
文献类型：
--
作者：
Shimaji K;Tanaka R;Maeda T;Ozaki M;Yoshida H;Ohkawa Y;Sato T;Suyama M;Yamaguchi M
生物体已经发展出保护自己免受饥饿压力的行为策略。尽管它们在自然界中很重要，但人们对其潜在机制知之甚少。在这里，我们发现果蝇G9a （dG9a）是组蛋白H3 Lys 9特异性组蛋白甲基转移酶之一，在饥饿诱导行为中起关键调节作用。利用dG9a零突变果蝇进行的rna测序分析显示，饥饿条件下dG9a调控了一些与味觉感知相关的基因的表达。反转录定量pcr分析表明，饥饿dG9a零突变体中糖感味觉受体基因表达上调。与此一致的是，长鼻伸展反射试验表明，dG9a的消耗增加了饥饿条件下对蔗糖的敏感性。此外，饥饿dG9a零突变体的运动活性也有所提高。我们还发现dG9a缺失下调胰岛素样肽基因的表达，而胰岛素样肽基因是抑制饥饿引起的多动所必需的。此外，野生型果蝇在饥饿状态后重新进食，恢复了过度活跃，增加了对蔗糖的敏感性，以及dG9a的表达水平。这些数据表明，dG9a是饥饿条件下行为策略决策的关键调节因子。
Organisms have developed behavioral strategies to defend themselves from starvation stress. Despite of their importance in nature, the underlying mechanisms have been poorly understood. Here, we show that Drosophila G9a (dG9a), one of the histone H3 Lys 9-specific histone methyltransferases, functions as a key regulator for the starvation-induced behaviors. RNA-sequencing analyses utilizing dG9a null mutant flies revealed that the expression of some genes relating to gustatory perception are regulated by dG9a under starvation conditions. Reverse transcription quantitative-PCR analyses showed that the expression of gustatory receptor genes for sensing sugar are up-regulated in starved dG9a null mutant. Consistent with this, proboscis extension reflex tests indicated that dG9a depletion increased the sensitivity to sucrose under starvation conditions. Furthermore, the locomotion activity was promoted in starved dG9a null mutant. We also found that dG9a depletion down-regulates the expression of insulin-like peptide genes that are required for the suppression of starvation-induced hyperactivity. Furthermore, refeeding of wild type flies after starvation conditions restores the hyperactivity and increased sensitivity to sucrose as well as dG9a expression level. These data suggest that dG9a functions as a key regulator for the decision of behavioral strategies under starvation conditions.
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影响因子：
2.1
作者：
Kato, Yasuko;Kato, Masaki;Yamaguchi, Masamitsu
通讯作者：
Yamaguchi, Masamitsu
影响因子：
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作者：
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通讯作者：
Hoch, Michael