Secondary structure model of the coat protein gene of turnip yellow mosaic virus RNA: Long, C-rich, single-stranded regions
Secondary structure model of the coat protein gene of turnip yellow mosaic virus RNA: Long, C-rich, single-stranded regions
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DOI：
10.1006/viro.1996.0505
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发表时间：
1996-10-01
期刊：
影响因子：
3.7
通讯作者：
Pleij, CWA
中科院分区：
文献类型：
--
作者：
Hellendoorn, K;Mat, AW;Pleij, CWA
所有的台风病毒属（一组ssRNA植物病毒）的RNA具有与大多数其他病毒不同的碱基组成。过量的胞嘧啶（35-42%）和低数量的鸟苷（15-17%）必然促使RNA结构具有相对低量的碱基配对和高发生率的未配对胞嘧啶。这些未配对的胞嘧啶可能在RNA-蛋白质相互作用中起作用。为了深入了解RNA在病毒粒子内的定位方式，通过结构探测、序列比较和计算机预测，已经确定了TYMV RNA的一部分的二级结构，包括所谓的tymobox、外壳蛋白基因和3'非翻译区。二级结构元件在台风病毒中的保守性不高，并且与一级结构的保守性不平行。然而，结构预测和探测实验的组合导致了由100-200个核苷酸的结构域散布着长的未配对的富含胞嘧啶的区域组成的模型。后者可与病毒粒子内的外壳蛋白相互作用。本文还讨论了TYMV RNA的3'端部分的一些功能区的结构。(C)出版社：Academic Press，Inc.
The RNA of all tymoviruses, a group of ssRNA plant viruses, has a base composition that is different from that of most other viruses. The excess of cytosines (35-42%) and the low number of guanosines (15-17%) must impel an RNA structure with a relatively low amount of base pairing and a high incidence of unpaired cytosines. These unpaired cytosines probably function in RNA-protein interactions. To gain insight into the way the RNA is positioned inside the virion, the secondary structure has been determined of a part of TYMV RNA, including the so-called tymobox, the coat protein gene, and the 3' untranslated region, by structure probing, sequence comparison, and computer predictions. Conservation of secondary structure elements in tymoviruses is not high and does not parallel the conservation of the primary structure. A combination of structure prediction and probing experiments, however, results in a model consisting of structured domains of 100-200 nucleotides interspersed by long unpaired cytosine-rich regions. The latter may interact with the coat protein inside the virion. The structure of some functionally interesting regions of the 3' part of TYMV RNA is also discussed. (C) 1996 Academic Press, Inc.