Development of methodology for the determination of carbon isotope ratios using gas chromatography/combustion/isotope ratio mass spectrometry and applications in the biodegradation of phenolic brominated flame retardants and their degradation products
Development of methodology for the determination of carbon isotope ratios using gas chromatography/combustion/isotope ratio mass spectrometry and applications in the biodegradation of phenolic brominated flame retardants and their degradation products
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开发使用气相色谱/燃烧/同位素比质谱测定碳同位素比的方法及其在酚类溴化阻燃剂及其降解产物的生物降解中的应用
DOI：
10.1002/rcm.7072
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发表时间：
2015
影响因子：
2
通讯作者：
Taicheng An
中科院分区：
文献类型：
--
作者：
Jukun Xiong;Guiying Li;Taicheng An
依据酚类溴化阻燃剂（BFR）和双酚A（BPA）的化合物特定稳定碳同位素分析已被证明可提供信息，以区分其在环境中的来源和归宿分配。然而，由于这些化合物含有高度极性的官能团，并在环境基质中以复杂的混合物形式存在，衍生化是一个必要的步骤，这会增加额外的非分析物碳原子用于分析，并可能改变原始的稳定碳同位素比。方法采用气相色谱/燃烧/同位素比值质谱（GC/C/IRMS）法测定BFR和BPAN，O-双（三甲基硅基）三氟乙酰胺（BSTFA）衍生物的δ 13 C值。用元素分析仪/同位素比值质谱法（EA/IRMS）测定了BSTFA试剂和标准未衍生化BFR和BPA的δ 13 C值。实验测得的BFRs和BPA衍生物的δ 13 C值与理论计算值进行了比较，结果表明，衍生化过程不会引起BFRs和BPA的同位素分馏（理论计算值与实验测得的δ 13 C值之间的平均差值为0.06 ± 0.15‰，在GC/C/IRMS测量的精度范围内）。因此，δ 13 C值的原始未衍生的BFRs和BPA的计算通过一个质量平衡equation.CONCLUSIONSThis工作提供了一个新的工具，研究生物或非生物转化过程中的BFRs和BPA在环境中，并将提供一个视角，为识别这些有机化合物的环境来源和归宿。版权所有© 2014约翰威利父子有限公司.
RATIONALECompound‐specific stable carbon isotope analysis of phenolic brominated flame retardants (BFRs) and bisphenol A (BPA) has proven informative to discriminate their source and fate apportionment in the environment. However, because these compounds contain highly polar functional groups and exist as complex mixtures in environment matrices, derivatization is a necessary step, which adds additional non‐analyte carbon atoms for analyses and may alter the original stable carbon isotope ratio. It is, therefore, imperative to gain an insight into the relationship between the δ13C values of the BFRs and BPA derivatives and those of underivatized BFRs and BPA.METHODSThe δ13C values of BFRs and BPAN,O‐bis(trimethylsilyl)trifluoroacetamide (BSTFA) derivatives were measured by gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). The δ13C values of the BSTFA reagent and the standard underivatized BFRs and BPA were determined using elemental analyzer/isotope ratio mass spectrometry (EA/IRMS). The experimentally obtained δ13C values for BFRs and BPA derivatives were then compared with the theoretically calculated values.RESULTSThe derivatization process introduces no isotopic fractionation for BFRs and BPA (the average difference between the theoretically calculated and experimentally obtained δ13C values was 0.06 ± 0.15‰, within the precision limits of the GC/C/IRMS measurements). Therefore, the δ13C values for the original underivatized BFRs and BPA were computed through a mass balance equation.CONCLUSIONSThis work offers a novel tool to research the biotic or abiotic transformation processes of BFRs and BPA in the environment and will offer a perspective for the identification of the environmental source and fate of these organic compounds. Copyright © 2014 John Wiley & Sons, Ltd.