The mobility of single-file water molecules is governed by the number of H-bonds they may form with channel-lining residues.
The mobility of single-file water molecules is governed by the number of H-bonds they may form with channel-lining residues.
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DOI：
10.1126/sciadv.1400083
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发表时间：
2015-03-20
期刊：
影响因子：
13.6
通讯作者：
Pohl P
中科院分区：
文献类型：
--
作者：
Horner A;Zocher F;Preiner J;Ollinger N;Siligan C;Akimov SA;Pohl P
由氢键决定的单列水分子的迁移率。通道几何形状根据经典流体动力学控制单一渗透水通道渗透率pf。然而，对于具有收缩区的膜通道，pf变化几个数量级，收缩区的宽度为一个水分子，长度为4至8个分子。我们发现，这些渠道的PF和单行沃茨在其中的扩散系数是由在通道壁中的残留物，可能形成氢键与单行沃茨的NH数。水扩散系数对NH的对数依赖性与较高NH密度下的结合选择的多样性一致。我们获得了高精度的pf值（i）通过荧光相关光谱法和高速原子力显微镜测量了囊泡膜中重组水通道蛋白的丰度，以及（ii）通过我们新改编的Rayleigh-Gans-Debye方程从散射光强度获得了囊泡水流出量。
Mobility of single-file water molecules determined by H-bonds. Channel geometry governs the unitary osmotic water channel permeability, pf, according to classical hydrodynamics. Yet, pf varies by several orders of magnitude for membrane channels with a constriction zone that is one water molecule in width and four to eight molecules in length. We show that both the pf of those channels and the diffusion coefficient of the single-file waters within them are determined by the number NH of residues in the channel wall that may form a hydrogen bond with the single-file waters. The logarithmic dependence of water diffusivity on NH is in line with the multiplicity of binding options at higher NH densities. We obtained high-precision pf values by (i) having measured the abundance of the reconstituted aquaporins in the vesicular membrane via fluorescence correlation spectroscopy and via high-speed atomic force microscopy, and (ii) having acquired the vesicular water efflux from scattered light intensities via our new adaptation of the Rayleigh-Gans-Debye equation.
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影响因子：
64.8
作者：
Gonen, T;Sliz, P;Walz, T
通讯作者：
Walz, T
影响因子：
4.8
作者：
Mathai, JC;Mori, S;Agre, P
通讯作者：
Agre, P
影响因子：
56.9
作者：
de Groot, BL;Grubmüller, H
通讯作者：
Grubmüller, H
影响因子：
8.6
作者：
Berezhkovskii, A;Hummer, G
通讯作者：
Hummer, G
DOI：
10.1016/0005-2736(74)90112-6
发表时间：
1974-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
LEVITT, DG;SUBRAMANIAN, G
通讯作者：
SUBRAMANIAN, G