Use of gas chromatography/mass spectrometry with positive chemical ionization for the determination of opiates in human oral fluid
Use of gas chromatography/mass spectrometry with positive chemical ionization for the determination of opiates in human oral fluid
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DOI：
10.1002/rcm.2449
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发表时间：
2006-01-01
影响因子：
2
通讯作者：
Fernández, P
中科院分区：
文献类型：
--
作者：
Cámpora, P;Bermejo, AM;Fernández, P
建立了同时测定人口服液中可待因、吗啡和6-乙酰吗啡（6AM）含量的分析方法。该方法采用液-液萃取法，以99:1 (v/v) N衍生化，o -双（三甲基硅基）三氟乙酰胺(BSTFA)/三甲基氯硅烷（TMCS），气相色谱/质谱法（GC/PCI-MS）测定。在30 ~ 500 ng/mL浓度范围内，检测器的响应呈线性关系，相关系数大于0.99。当变异系数小于7.5%时，精密度可接受。可待因检出限为0.7 ng/mL，吗啡检出限为2.0 ng/mL， 6AM检出限为0.6 ng/mL。将该方法应用于80份阿片类药物使用者的口服液样本，其中98%的样本对这三种分析物呈阳性。人口服液是一种适用于气相色谱- PCI-MS测定阿片类药物的生物液。版权所有(c) 2006约翰威利父子有限公司
An analytical method for the simultaneous determination of codeine, morphine and 6-acetylmorphine (6AM) in human oral fluid was developed. The method involves liquid-liquid extraction in Toxitubes A (R), derivatization with 99:1 (v/v) N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA)/trimethylchlorosilane (TMCS), and gas chromatography/mass spectrometry with positive chemical ionization (GC/PCI-MS) determination. The detector response was linear over the concentration range 30-500 ng/mL with coefficients of correlation higher than 0.99. The precision was acceptable with coefficients of variation less than 7.5%. The limits of detection achieved were 0.7 ng/mL for codeine, 2.0 ng/mL for morphine, and 0.6 ng/mL for 6AM. The method proposed was applied to 80 oral fluid samples from opiates users, 98% of which were positive for the three analytes. Human oral fluid is a suitable biological fluid for the determination of opiates by GC/PCI-MS. Copyright (c) 2006 John Wiley & Sons, Ltd.