Acute effects of ethanol on biosynthesis and glycosylation of IgGl(kappa) antibody molecules in cultured P3/X63-Ag8 myeloma cells.
Acute effects of ethanol on biosynthesis and glycosylation of IgGl(kappa) antibody molecules in cultured P3/X63-Ag8 myeloma cells.
复制标题
乙醇对培养的P3/X63-Ag8骨髓瘤细胞中IgG1(κ)抗体分子的生物合成和糖基化的急性影响。
DOI：
--
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
L. Ingram
中科院分区：
文献类型：
--
作者：
L. Eaton;L. Ingram
急性乙醇暴露可抑制培养的P3/X63-Ag8小鼠骨髓瘤细胞蛋白质合成。然而，IgG1抗体的合成占总蛋白质合成的百分比略有增加。使用放线菌素D的实验表明，乙醇对蛋白质合成的全面抑制发生在翻译水平。在L-[35S]蛋氨酸脉冲刺激下，培养的P3/X63-Ag8细胞含有一个轻抗体多肽和两个重抗体多肽。这些重链中的一个被证明是另一个成熟分子的未糖化的前体。只有糖基化的多肽才是分泌的IgG1抗体的正常成分。在暴露于乙醇(0.1v/v%及以上)的细胞中，在1小时的同位素脉冲中，未成熟重链的糖基化比未暴露的对照细胞发生得更快。观察到酒精对抗体糖基化的影响可能与酒精患者对感染的易感性增加有关。乙醇还可能影响骨髓瘤细胞和其他组织中其他糖蛋白的合成。
Protein synthesis in cultured P3/X63-Ag8 mouse myeloma cells was inhibited by acute exposure to ethanol. However, the synthesis of IgGl antibody, as a percentage of total protein synthesis, increased slightly. Experiments using actinomycin D suggest that the overall inhibition of protein synthesis by ethanol occurs at the translational level. Following an L-[35S]methionine pulse, cultured P3/X63-Ag8 cells contained one light antibody polypeptide and two heavy antibody polypeptides. One of these heavy chains was shown to be the unglycosylated precursor of the other, mature molecule. Only the glycosylated polypeptide is a normal constituent of secreted IgGl antibody. The glycosylation of the immature heavy chains occurred more rapidly during a 1-hr isotopic pulse in cells exposed to ethanol (0.1 v/v % and above), than in unexposed control cells. The observed effects of ethanol on antibody glycosylation may be related to the increased susceptibility of alcoholic patients to infections. Ethanol may also affect the synthesis of other glycoproteins in myeloma cells and other tissues.