Role of the actin cytoskeleton during influenza virus internalization into polarized epithelial cells
Role of the actin cytoskeleton during influenza virus internalization into polarized epithelial cells
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DOI：
10.1111/j.1462-5822.2007.00900.x
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发表时间：
2007-07-01
影响因子：
3.4
通讯作者：
Whittaker, Gary R.
中科院分区：
文献类型：
--
作者：
Sun, Xiangjie;Whittaker, Gary R.
流感病毒在体内的感染部位是极化上皮，并且已经确定病毒优先从极化上皮细胞的顶端表面进入；然而，在病毒内吞进入极化上皮的过程中涉及的许多分子事件仍不清楚。在这里，我们研究了肌动蛋白微丝和肌球蛋白VI运动蛋白在流感进入极化和非极化细胞时的作用。通过细胞松弛素D和茉莉素内酯处理细胞，我们发现流感病毒进入所有极化上皮细胞都需要肌动蛋白动力学，在病毒从质膜内化的过程中，肌动蛋白细胞骨架起着特定的作用。相比之下，在破坏或稳定肌动蛋白细胞骨架后，流感病毒仍然可以有效地进入并感染所有非极化细胞。为了检验肌动蛋白运动蛋白myosin VI的作用，我们在极化和非极化细胞中表达了一个显性阴性结构。流感病毒在myosin VI尾部突变体转染细胞中的传染性在极化上皮细胞中显著降低，而在非极化细胞中则没有显著降低。总的来说，我们的数据表明动态肌动蛋白细胞骨架在流感病毒进入极化上皮细胞中起着不可或缺的作用，这是非极化细胞所不具有的特征。
The in vivo site of influenza virus infection is a polarized epithelium, and it is well established that the virus preferentially enters from the apical surface of polarized epithelial cells; however, many of the molecular events involved during the endocytosis of the virus into polarized epithelia remain unclear. Here we examined the role of actin microfilaments and the myosin VI motor protein during influenza entry into a panel of polarized and non-polarized cells. By treatment of cells with cytochalasin D and jasplakinolide, we show that influenza virus entry into all the polarized epithelial cells tested requires actin dynamics, with a specific role for the actin cytoskeleton in the process of virus internalization from the plasma membrane. In contrast, influenza could still could efficiently enter and infect all non-polarized cells tested after disruption or stabilization of the actin cytoskeleton. To examine the role of the actin motor protein, myosin VI, we expressed a dominant-negative construct in both polarized and non-polarized cells. Influenza virus infectivity in myosin VI tail mutant-transfected cells was significantly decreased in polarized epithelial cells, but not in non-polarized cells. As a whole, our data suggest indispensable roles of a dynamic actin cytoskeleton for influenza virus entry into polarized epithelial cells, a feature not shared with non-polarized cells.