Changes in the blood routine, biochemical indexes and the pro-inflammatory cytokine expressions of peripheral leukocytes in postpartum dairy cows with metritis
Changes in the blood routine, biochemical indexes and the pro-inflammatory cytokine expressions of peripheral leukocytes in postpartum dairy cows with metritis
复制标题
产后子宫炎奶牛血常规、生化指标及外周血促炎细胞因子表达的变化
DOI：
10.1186/s12917-019-1912-y
复制
发表时间：
2019-05-21
影响因子：
2.6
通讯作者：
Li, Jianji
中科院分区：
文献类型：
--
作者：
Cui, Luying;Wang, Heng;Li, Jianji
BackgroundThe的目的，本研究的目的是澄清全血细胞计数，血液生化，外周白色血细胞的促炎细胞因子的基因表达在产后奶牛子宫炎.ResultsThe奶牛被分配到对照组（n= 28）或子宫炎组（n= 28），回顾性。在估计分娩前7天（-7天）、分娩当天（0天）、分娩后7天和30天采集血样。-7 d时，对照组和子宫炎组之间的血液指标无差异。WBC、粒细胞和单核细胞在第7和30 d时均高于对照组。与对照组相比，所有肝功能参数和甘油三酯水平在0，7和30天，肌酐水平在7和30天的奶牛子宫炎。与对照组奶牛相比，子宫炎组奶牛在0、7和30 d时的Ca和P浓度以及0 d时的葡萄糖浓度均较低。其中30 d时WBC、7 d时AST均超过正常范围（WBC：5.0 ~ 16.0 × 109/L; AST：42.5 ~ 98 U/L），而葡萄糖和钙在0 ~ 30 d均低于正常范围（葡萄糖：2.5 ~ 4.5mmol/L;钙：2.2 ~ 2.5mmol/L）。子宫炎组促炎细胞因子的基因表达高于对照组，包括IL-1α在第7天，IL-1β在第-7、0和7天，IL-6在第-7、0、7和30天，IL-8在第0、7和30天，结论子宫炎奶牛产犊后4周出现全身炎症反应，肝功能损害，代谢状态改变，甘油三酯水平升高，葡萄糖、钙和磷浓度降低。
BackgroundThe aim of the present study was to clarify the changes in complete blood count, blood biochemistry, and the gene expressions of pro-inflammatory cytokines of peripheral white blood cells in postpartum dairy cows with metritis.ResultsThe cows were assigned to the control group (n= 28) or the metritis group (n= 28), retrospectively. Blood samples were taken 7 days before the estimated parturition (− 7 d), on the day of parturition (0 d), and 7 and 30 d after parturition. There was no difference in blood indexes between the control group and the metritis group at − 7 d. The WBC, granulocytes and monocytes were generally higher at 7 and 30 d in the metritis group than the control. In comparison with the controls, all liver function parameters and triglyceride levels at 0, 7 and 30 d, and the creatinine level at 7 and 30 d were higher in cows with metritis. The concentrations of Ca and P at 0, 7 and 30 d, and of glucose at 0 d were lower for cows in the metritis group compared with cows in the control group. Among these parameters, the WBC at 30 d, the aspartate aminotransferase activity (AST) at 7 d exceeded normal ranges (WBC: 5.0 ~ 16.0 × 109/L; AST: 42.5 ~ 98 U/L), whereas the concentrations of glucose and Ca from 0 to 30 d were below normal ranges (glucose: 2.5 ~ 4.5 mmol/L; Ca: 2.2 ~ 2.5 mmol/L) in the metritis group. The gene expressions of pro-inflammatory cytokines in the metritis group were higher than those in the control group, including the IL-1α at 7d, the IL-1β at − 7, 0 and 7 d, the IL-6 at − 7, 0, 7 and 30 d, the IL-8 at 0, 7 and 30 d, and the TNF-α at 7 and 30 d.ConclusionThe cows with metritis experienced systemic inflammation for 4 weeks after calving, the impaired hepatic function, and the altered metabolic status with increased triglyceride level and decreased concentrations of glucose, Ca and P.