Soybean oil alleviates maternal conjugated linoleic acid dietary-induced hatchability decrease and embryo hepatic lipolysis in broiler breeders
Soybean oil alleviates maternal conjugated linoleic acid dietary-induced hatchability decrease and embryo hepatic lipolysis in broiler breeders
复制标题
豆油可缓解母体共轭亚油酸饮食引起的肉种鸡孵化率下降和胚胎肝脂肪分解
DOI：
10.1016/j.livsci.2019.103913
复制
发表时间：
2020-02-01
影响因子：
1.8
通讯作者：
Liu, Xuelan
中科院分区：
文献类型：
--
作者：
Fu, Chunyan;Zhang, Yan;Liu, Xuelan
The objective of the present study was to determine the effect of maternal conjugated linoleic acid (CLA) and soybean oil (SO) dietary on egg hatchability and hepatic lipometabolism in chick embryos. Broiler breeders (36 wk, 3.56 +/- 0.12 kg) were randomly assigned to 6 treatments (with 6 replicates per treatment and 25 broiler breeder hens and 1 rooster per replicate) and fed a diet with 0.5% SO (control), or a diet with either 0.5% CLA, or CLA plus 0, 2, 4, 6, or 8% SO for 8 wk, with natural mating. The results showed that 0.5% CIA reduced hatchability of fertile eggs (P < 0.0001), which was reversed by SO supplementation and fully recovered at 6% SO (linear, P < 0.0001; quadratic, P < 0.0001). Meanwhile, maternal CIA dietary decreased the mRNA expression level of malic enzyme, sterol regulatory element-binding protein-1c (SREBP-1c), fatty acid synthetase and acetyl-CoA carboxylase 1 (ACC1) (P < 0.05), and increased the expression of peroxisome proliferator-activated receptor alpha (PPAR alpha), and carnitine palmitoyl transferase 1 (CPT1) (P < 0.05) in the embryonic liver on day 14 and 21. In addition, the protein expression level of phospho-ACC1, phospho-PPAR alpha and CPT1 were increased by maternal CIA dietary (P < 0.05), and the protein expression of phospho-SREBP-1c was inhibited (P < 0.05), indicating that maternal CIA dietary reduced fatty acid (FA) synthesis and accelerated FA catabolism. Soybean oil addition diminished CIA-mediated fatty acid catabolism, via increasing the gene and protein expression of fatty acid synthesis (ME, SREBP-1c, FAS, ACC1) (Linear, P < 0.05) but decreasing that of fatty acid lipolysis (PPAR alpha and CPT1) (Linear, P < 0.05). In conclusion, SO supplementation modulated maternal CIA dietary-induced loss in egg hatchability and hepatic lipolysis in embryo livers. The regulatory mechanism of maternal CIA and SO dietary in offspring lipometabolism might be related to SREBP-1c and PPAR-alpha signaling pathways.
