Fast folding of a helical protein initiated by the collision of unstructured chains
Fast folding of a helical protein initiated by the collision of unstructured chains
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DOI：
10.1073/pnas.0404057101
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发表时间：
2004-09-14
影响因子：
11.1
通讯作者：
Sosnick, TR
中科院分区：
文献类型：
--
作者：
Meisner, WK;Sosnick, TR
为了检验螺旋的形成是否必然先于链碰撞，我们测量了一个经过特殊设计的全螺旋线圈的折叠，该线圈具有可以忽略的内在螺旋倾向，但总体稳定性很高。折叠速度接近扩散限制值，如果折叠取决于碰撞前螺旋的形成，则折叠速度比可能的要快得多。因此，两条非结构链的碰撞是占优势的动力学途径的初始步骤，而螺旋度只在较晚的阶段起作用。从非结构化的遭遇复合体中折叠可能是有效和健壮的，这对任何将折叠和结合结合起来的生物过程都有影响。
To examine whether helix formation necessarily precedes chain collision, we have measured the folding of a fully helical coiled coil that has been specially engineered to have negligible intrinsic helical propensity but high overall stability. The folding rate approaches the diffusion-limited value and is much faster than possible if folding is contingent on precollision helix formation. Therefore, the collision of two unstructured chains is the initial step of the dominant kinetic pathway, whereas helicity exerts its influence only at a later step. Folding from an unstructured encounter complex may be efficient and robust, which has implications for any biological process that couples folding to binding.