Dynamic expression of the basic helix-loop-helix transcription factor NeuroD in the rod and cone photoreceptor lineages in the retina of the embryonic and larval zebrafish
Dynamic expression of the basic helix-loop-helix transcription factor NeuroD in the rod and cone photoreceptor lineages in the retina of the embryonic and larval zebrafish
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DOI：
10.1002/cne.21150
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发表时间：
2007-03-01
影响因子：
2.5
通讯作者：
Hitchcock, Peter F.
中科院分区：
文献类型：
--
作者：
Ochocinska, Malgorzata J.;Hitchcock, Peter F.
NeuroD 是一种基本的螺旋-环-螺旋 (bHLH) 转录因子，对于确定神经元细胞命运和调节细胞周期退出至关重要。我们之前表明，在金鱼中，neuroD 在杆状感光细胞谱系中表达，并且我们推断，neuroD 也在无长突细胞和新生视锥细胞感光细胞的子集中表达。在这里，我们通过检查胚胎和幼虫斑马鱼中neuroD的时间和空间表达模式以及通过识别表达该基因的细胞类型来扩展该研究。 NeuroD 在发育中的斑马鱼视网膜中的表达是动态的，跨越早期视网膜发生和视锥细胞感光器的成熟。在早期视网膜发生中，neuroD 表达从腹鼻视网膜中的一小块扩展到剩余的视网膜神经上皮。随着视网膜发生的进展，neuroD 的表达变得仅限于无长突细胞、未成熟的视锥细胞以及视杆细胞和视锥细胞谱系的细胞。这种表达在受精后 96 小时 (hpf) 时达到成体模式，随后视网膜中央神经 D 表达的时间模式在萌发边缘进行空间重现。细胞表达模式表明，neuroD 调节视杆细胞和视锥细胞发生的各个方面，但通过不同的细胞谱系。此外，neuroD 与视锥细胞祖细胞和新生视锥光感受器中的视锥-杆同源框转录因子 (Crx) 共表达，这表明在斑马鱼视网膜中，与其他脊椎动物视网膜一样，类似的遗传级联调节光感受器的发生和成熟。
NeuroD is a basic helix-loop-helix (bHLH) transcription factor critical for determining neuronal cell fate and regulating withdrawal from the cell cycle. We showed previously that, in goldfish, neuroD is expressed in the rod photoreceptor lineage, and we inferred that neuroD is also expressed in a subset of amacrine cells and nascent cone photoreceptors. Here we extended that study by examining the temporal and spatial expression pattern of neuroD in the embryonic and larval zebrafish and by identifying the cell types that express this gene. NeuroD expression in the developing zebrafish retina is dynamic, spanning early retinogenesis and the maturation of cone photoreceptors. In early retinogenesis neuroD expression expands from a small patch in the ventronasal retina, through the remaining retinal neuroepithelium. As retinogenesis progresses, neuroD expression becomes restricted to amacrine cells, immature cones, and cells of rod and cone lineages. This expression achieves an adult pattern by 96 hours postfertilization (hpf), whereupon the temporal pattern of neuroD expression in central retina is spatially recapitulated at the germinative margin. The cellular pattern of expression suggests that neuroD regulates aspects of rod and cone genesis, but through separate cellular lineages. Furthermore, neuroD is coexpressed with the cone-rodhomeobox transcription factor (Crx) in putative cone progenitors and nascent cone photoreceptors, suggesting that, in the zebrafish retina, as in other vertebrate retinas, similar genetic cascades regulate photoreceptor genesis and maturation.