Carcinogenicity of N-methyl-N-nitrosourea and N-ethyl-N-nitrosourea when applied to a localized area of the hamster trachea.
Carcinogenicity of N-methyl-N-nitrosourea and N-ethyl-N-nitrosourea when applied to a localized area of the hamster trachea.
复制标题
N-甲基-N-亚硝基脲和 N-乙基-N-亚硝基脲应用于仓鼠气管局部区域时的致癌性。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
R. Moon
中科院分区：
文献类型：
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作者：
C. Grubbs;P. J. Becci;H. Thompson;R. Moon
采用仓鼠气管肿瘤模型系统研究了n -甲基-n -亚硝基脲（MNU）和n -乙基-n -亚硝基脲（ENU）的致癌性。仓鼠接受15或10次每周一次的0.5或0.25% MNU溶液治疗，并在第一次气管内灌注9个月后被杀死。另外15只仓鼠接受每周一次的0.5、0.25或0.125%的ENU溶液治疗，6个月时处死。用MNU治疗导致气管癌的剂量依赖性诱导；94%的诱导肿瘤为表皮样癌和腺癌合并。用0.5、0.25、0.125%的ENU溶液处理仓鼠分别诱导良性气管肿瘤（乳头状瘤和息肉）的发生率分别为83%、64%和71%。未见ENU诱导气管癌变。证实了MNU的致癌性和局部气管冲洗肿瘤模型系统诱导肿瘤的可重复性。此外，局部气管清洗技术对检测化合物对呼吸道上皮的致瘤性的敏感性也得到了证实。
The carcinogenicity of N-methyl-N-nitrosourea (MNU) and N-ethyl-N-nitrosourea (ENU) was studied with the use of a hamster tracheal tumor model system. Hamsters received 15 or 10 once-weekly treatments of either a 0.5 or 0.25% solution of MNU and were killed 9 months after the first intratracheal instillation. Other hamsters received 15 once-weekly treatments of a 0.5, 0.25, or 0.125% solution of ENU and were killed at 6 months. Treatment with MNU resulted in a dose-dependent induction of tracheal carcinomas; 94% of the tumors induced were combined epidermoid and adenocarcinomas. Treatment of hamsters with a 0.5, 0.25, 0.125% solution of ENU induced an 83, 64, and 71% incidence of benign tracheal tumors, respectively (papillomas and polyps). No tracheal carcinomas were induced by ENU. The carcinogenicity of MNU and the reproducibility of tumor induction with the use of the localized tracheal washing, tumor model system were confirmed. Furthermore, the sensitivity of the localized tracheal washing technique for the detection of the tumorigenicity of compounds toward respiratory epithelium was demonstrated.