SIMPLE TECHNIQUE FOR DETECTION OF DENGUE ANTIGEN IN MOSQUITOS BY IMMUNOFLUORESCENCE

SIMPLE TECHNIQUE FOR DETECTION OF DENGUE ANTIGEN IN MOSQUITOS BY IMMUNOFLUORESCENCE
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DOI:
10.4269/ajtmh.1977.26.533
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发表时间:
1977-01-01
影响因子:
3.3
通讯作者:
ROSEN, L
ROSEN, L
中科院分区:
医学4区
文献类型:
--
作者:
KUBERSKI, TT;ROSEN, L

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用直接荧光抗体技术检测感染4种登革热血清型的白纹伊蚊雄蚊或雌蚊的简单头标本,均能检测到登革抗原。通过将该技术与用试验材料对蚊子进行肠道外接种的技术相结合,可以快速而简单地分离登革病毒。
Dengue antigen could be demonstrated by a direct fluorescent antibody technique in simple head squashes of male or female Aedes albopictus mosquitoes infected with any of the 4 dengue serotypes. Dengue viruses can be isolated rapidly and simply by combining this technique with that of parenteral inoculation of mosquitoes with test material.