Comparative Study of Nonautolytic Mutant and Wild-Type Strains of Coprinopsis cinerea Supports an Important Role of Glucanases in Fruiting Body Autolysis
Comparative Study of Nonautolytic Mutant and Wild-Type Strains of Coprinopsis cinerea Supports an Important Role of Glucanases in Fruiting Body Autolysis
复制标题
灰鬼伞非自溶突变体和野生型菌株的比较研究支持葡聚糖酶在子实体自溶中的重要作用
DOI：
10.1021/acs.jafc.5b03962
复制
发表时间：
2015-11-04
影响因子：
6.1
通讯作者：
Yuan, Sheng
中科院分区：
文献类型：
--
作者：
Liu, Zhonghua;Niu, Xin;Yuan, Sheng
Autolysis of Coprinopsis cinerea fruiting bodies affects its commercial value. In this study, a mutant of C. cinerea that exhibits pileus expansion without pileus autolysis was obtained using ultraviolet mutagenesis. This suggests that pileus expansion and pileus autolysis involve different enzymes or proteins. Among the detected hydrolytic enzymes, only beta-1,3-glucanase activity increased with expansion and autolysis of pilei in the wild-type strain, but the increase was abolished in the mutant. This suggests that beta-1,3-glucanases plays a major role in the autolysis. Although there are 43 possible beta-1,3-glucoside hydrolases genes, only 4 known genes, which have products that are thought to act synergistically to degrade the beta-1,3-glucan backbone of cell walls during fruiting body autolysis, and an unreported gene were upregulated during pileus expansion and autolysis in the wild-type stain but were suppressed in the mutant. This suggests that expression of these beta-1,3-glucanases is potentially controlled by a single regulatory mechanism.
