Anticonvulsant effect of carnosine on penicillin-induced epileptiform activity in rats
Anticonvulsant effect of carnosine on penicillin-induced epileptiform activity in rats
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DOI：
10.1016/j.brainres.2008.08.019
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发表时间：
2008-11-06
期刊：
影响因子：
2.9
通讯作者：
Bagirici, Faruk
中科院分区：
文献类型：
--
作者：
Kozan, Ramazan;Sefil, Fatih;Bagirici, Faruk
肌肽是由β-丙氨酸和L组氨酸通过肌肽合成酶合成的天然二肽化合物。最近的报道称，肌肽在癫痫的控制中起着重要的作用，但它在抗癫痫功能中的作用尚不清楚。在这项研究中，我们使用皮质内注射青霉素的方法研究了肌肽在癫痫大鼠模型中的作用。分别于注射青霉素后30min和注射前90min分别给予125、250、500、1000 mg/kg肌肽和500 mg/kg肌肽。皮层脑电(ECoG)记录证实癫痫样活动。与注射青霉素的大鼠相比，所有肌肽处理组大鼠的青霉素诱发癫痫样活动的平均峰频率均显著降低。肌肽500 mg/kg剂量对青霉素致痫大鼠癫痫发作的抑制作用最强。青霉素500 mg/kg组与青霉素500 mg/kg组相比，平均癫痫发作时间差异无统计学意义。提示肌肽对青霉素诱导的大鼠癫痫有抗惊厥作用。因此，我们的数据支持肌肽可能是一种潜在的抗癫痫药物在未来的临床治疗癫痫的假设。(C)2008爱思唯尔B.V.保留所有权利。
Carnosine is a compound of naturally-occurring dipeptide that synthesized by the carnosine synthetase from beta-alanine and L-histidine. Recent reports claim that carnosine plays an important role in the control of epilepsy but its involvement in anticonvulsant functions remains unknown. In this study, we investigated the effects of carnosine in a rat model of epilepsy using the intracortical penicillin injection method. Thirty minutes after penicillin injection, the doses of 125, 250, 500, 1000 mg/kg carnosine and 90 min before penicillin injection the dose of 500 mg/kg carnosine were administered intraperitoneally. The epileptiform activity was verified by electrocorticographic (ECoG) recordings. The mean spike frequency of penicillin-induced epileptiform activity was significantly decreased in all carnosine-treated rats when compared with those of penicillin-injected. The dose of 500 mg/kg for carnosine treated and pretreated rats was found to be the most effective dose in reducing the frequency of penicillin-induced epileptiform activity. There was no significant difference in the mean onset of epileptiform activity between penicillin and 500 mg/kg camosine pretreated groups. These findings indicate that carnosine has an anticonvulsant effect on penicillin-induced epilepsy in rats. Thus, our data support the hypothesis that carnosine may be a potential anticonvulsant drug for clinical therapy of epilepsy in the future. (C) 2008 Elsevier B.V. All rights reserved.