Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue
Evidence for an immunological relationship between Streptococcus mutans and human cardiac tissue
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变形链球菌与人类心脏组织之间免疫学关系的证据
作者：
Michael Hughes;M. Susan;Machardy;'. A. J. Sheppard;C. Nicholas;Woods
用双向免疫电泳、间接免疫荧光和放射免疫分析等方法证明，某些变形链球菌株免疫兔的抗血清中含有与人心肌组织发生交叉反应的抗体。这些兔子被致敏到一个令人震惊的剂量的人类心脏抗原，过敏性死亡，有时产生。心肌炎也是免疫程序的结果。所有五种技术获得的数据具有可比性。交叉反应性可能与三种抗原ID、IF和HL相关。ID和IF抗原是S. Ingbritt，但HL抗体仅在超免疫后产生。交叉反应性具有免疫学性质，而不是非特异性因素（如细菌Fc反应组分或生长培养基成分引发的抗体）的结果。这些发现支持了S.变异体可诱导自身免疫反应，表明在配制任何龋齿疫苗之前必须谨慎选择抗原。
Two-dimensional immunoelectrophoresis, indirect immunofluorescence, and radioimmunoassay were used to demonstrate that antisera from rabbits immunized with some strains of Streptococcus mutans contain antibodies that cross-react with human cardiac tissue. These rabbits were sensitized to a shocking dose of human heart antigen, and anaphylactic deaths were sometimes produced. Myocarditis was also a result of the immunization procedure. Data obtained with all five techniques were comparable. Cross-reactivity could be associated with three antigens designated ID, IF, and HL. Antigens ID and IF were major immunogens of S. mutans Ingbritt, but HL antibodies were produced only after hyperimmunization. Corss-reactivity was of an immunological nature and not the result of nonspecific factors such as bacterial Fc reactive components or antibody elicited to growth medium constituents. These findings support the hypothesis that immunization with S. mutans can induce autoimmune reactions and indicate that antigens must be selected with caution before formulating any dental caries vaccine.