Expression of nucleotide P2X receptor subtypes during spermatogenesis in the adult rat testis
Expression of nucleotide P2X receptor subtypes during spermatogenesis in the adult rat testis
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DOI：
10.1159/000047905
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Burnstock, G
中科院分区：
文献类型：
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作者：
Glass, R;Bardini, M;Burnstock, G
使用针对所有七种 P2X 受体亚型的抗体，通过免疫组织化学和蛋白质印迹法研究成年大鼠睾丸中 ATP 门控离子通道（P2X 受体）的表达。在生殖细胞成熟过程中鉴定并监测免疫反应细胞。免疫组化和Western blotting实验结果显示，睾丸中表达P2X(1)、P2X(2)、P2X(3)、P2X(5)和P2X(7)受体，而P2X(4)和P2X(6)受体缺失。血管显示 P2X(1) 和 P2X(2) 受体免疫染色； P2X(1) 受体仅存在于血管中。发现 P2X(2)、P2X(3) 和 P2X(5) 受体在生精上皮周期的不同阶段的各种生殖细胞类型中存在差异表达。 P2X(2) 和P2X(3) 受体总是在相同细胞类型和相同阶段同时观察到。支持细胞在生精上皮周期期间还表现出 P2X(2) 和 P2X(3) 受体的差异染色，而 P2X(7) 受体表达存在于整个阶段。在 Leydig 细胞上未检测到 P2X 受体的免疫染色。讨论了嘌呤能信号在控制生殖细胞成熟中的可能作用。特别是，有人认为嘌呤能信号传导可能在控制成人睾丸中彼此并存的不同发育年龄的生殖细胞亚群的成熟中发挥作用。版权所有 (C) 2001 S. Karger AG，巴塞尔。
The expression of ATP-gated ion channels (P2X receptors) was investigated in testes of adult rats by immunohistochemistry and Western blotting with antibodies against all seven P2X receptor subtypes. Immunoreactive cells were identified and monitored during germ cell maturation. Results of immunohistochemical and Western blotting experiments showed the expression of P2X(1), P2X(2), P2X(3), P2X(5) and P2X(7) receptors, while P2X(4) and P2X(6) receptors were absent from the testis. Blood vessels displayed immunostaining for P2X(1) and P2X(2) receptors; the P2X(1) receptors were present exclusively in blood vessels. P2X(2), P2X(3) and P2X(5) receptors were found to be expressed differentially in the various germ cell types throughout the different stages of the cycle of the seminiferous epithelium; P2X(2) and P2X(3) receptors were always observed together in the same cell types and at the same stages. Sertoli cells also showed differential staining for P2X(2) and P2X(3) receptors during the cycle of the seminiferous epithelium, whereas P2X(7) receptor expression was present throughout all stages. No immunostaining for P2X receptors was detected on Leydig cells. The possible roles of purinergic signalling in the control of germ cell maturation are discussed. In particular, it is suggested that purinergic signalling may play a role in controlling the maturation of germ cell subsets of different developmental ages that exist alongside each other in the adult testis. Copyright (C) 2001 S. Karger AG, Basel.