GAS-LIQUID-CHROMATOGRAPHY OF THE GINGIVAL FLUID AS AN AID IN PERIODONTAL DIAGNOSIS
GAS-LIQUID-CHROMATOGRAPHY OF THE GINGIVAL FLUID AS AN AID IN PERIODONTAL DIAGNOSIS
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DOI：
10.1111/j.1600-0765.1985.tb00827.x
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发表时间：
1985-01-01
影响因子：
3.5
通讯作者：
BLASI, G
中科院分区：
文献类型：
--
作者：
BOTTA, GA;RADIN, L;BLASI, G
越来越多的证据表明，属于黑色素类杆菌的杆状革兰氏阴性厌氧菌可能在牙周病的发病机制中发挥作用。众所周知，厌氧细菌会产生几种挥发性脂肪酸作为其代谢的最终产物，通过直接气相色谱 (GLC) 在临床样本中检测这些脂肪酸已被有效地用来证明它们在感染部位的存在。本文报告了通过 GLC 分析从患有牙周病的患者身上收集的牙龈液所获得的结果。已发现 GLC 谱与牙龈指数和每个选定位点的革兰氏阴性菌定植具有良好的统计相关性。获得的数据强烈表明丁酸可能被认为是疾病严重程度的可靠标志物，其定量变化可能有助于跟踪该过程的临床过程。这些数据与丁酸的生物活性密切相关，据报道，丁酸在牙菌斑中含量丰富，并对包括牙龈成纤维细胞在内的多种细胞发挥细胞毒活性。尚未发现牙龈液流速与 GLC 中的脂肪酸谱之间存在相关性。由此可见，GLC衡量的是龈下区域菌群的整体代谢活性，而龈液流量则反映了宿主炎症反应的程度。两种实验室测试的结合使用应该为临床医生提供更多定量和可重复的参数来监测疾病活动。
There is increasing evidence that rod-shaped Gram negative anaerobic bacteria, as those belonging to black-pigmented Bacteroids, may play a role in the pathogenesis of periodontal diseases. Anaerobic bacteria are known to produce, as end-products of their metabolism several volatile fatty acids, whose detection in clinical specimens by direct gas liquid chromatography (GLC) has been usefully utilized to demonstrate their presence in the infected site. The results obtained analyzing by GLC the gingival fluid collected from patients suffering from periodontal diseases are reported here. A good statistical correlation of the GLC profile has been found with the gingival index and the colonization by gram-negative bacteria at each selected site. The data obtained strongly suggest that butyric acid may be considered a reliable marker of the severity of the disease, and its quantitative changes may be useful in following the clinical course of the process. The data correlate well with the biological activities of butyric acid, which have been reported to be abundant in plaque and to exert a cytotoxic activity on a variety of cells including gingival fibroblasts. No correlation has been found between gingival fluid flow rate and fatty acids profiles in GLC. It can be concluded that GLC measures the overall metabolic activity of the bacterial population colonizing the subgingival area, while the rate of gingival fluid flow reflects the degree of the inflammatory response of the host. The combined use of the two laboratory tests should provide the clinician with more quantitative and reproducible parameters for monitoring the disease activity.