Induction of neurite outgrowth through contactin and Nr-CAM by extracellular regions of glial receptor tyrosine phosphatase beta.
Induction of neurite outgrowth through contactin and Nr-CAM by extracellular regions of glial receptor tyrosine phosphatase beta.
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DOI：
10.1083/jcb.136.4.907
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发表时间：
1997-02-24
期刊：
影响因子：
--
通讯作者：
Grumet M
中科院分区：
文献类型：
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作者：
Sakurai T;Lustig M;Nativ M;Hemperly JJ;Schlessinger J;Peles E;Grumet M
受体蛋白酪氨酸磷酸酶 β (RPTPβ) 表达为可溶性受体形式，具有常见的细胞外区域，包括碳酸酐酶结构域 (C)、纤连蛋白 III 型重复序列 (F) 和称为 S 的独特区域。我们之前表明，具有 C 结构域 (βC) 的重组 Fc 融合蛋白与接触蛋白结合，支持神经元粘附和神经突生长。作为底物，βCFS 在支持细胞粘附方面效果较差，但它是比 βCF 更有效的神经突生长促进剂。 βS 本身没有作用，但与 βCF 混合时可增强神经突生长。 βCFS 诱导的神经突生长受到 Nr-CAM 和 contactin 抗体的抑制，这些细胞粘附分子形成了结合 βCFS 的复合物。转染并在其表面表达 βCFS 的 NIH3T3 细胞在培养物中诱导神经元分化。这些结果表明神经胶质 RPTPβ 与 contactin/Nr-CAM 复合物的结合对于神经突生长和神经元分化很重要。
Receptor protein tyrosine phosphatase β (RPTPβ) is expressed as soluble and receptor forms with common extracellular regions consisting of a carbonic anhydrase domain (C), a fibronectin type III repeat (F), and a unique region called S. We showed previously that a recombinant Fc fusion protein with the C domain (βC) binds to contactin and supports neuronal adhesion and neurite growth. As a substrate, βCFS was less effective in supporting cell adhesion, but it was a more effective promoter of neurite outgrowth than βCF. βS had no effect by itself, but it potentiated neurite growth when mixed with βCF. Neurite outgrowth induced by βCFS was inhibited by antibodies against Nr-CAM and contactin, and these cell adhesion molecules formed a complex that bound βCFS. NIH3T3 cells transfected to express βCFS on their surfaces induced neuronal differentiation in culture. These results suggest that binding of glial RPTPβ to the contactin/Nr-CAM complex is important for neurite growth and neuronal differentiation.