Printing chemical libraries on microarrays for fluid phase nanoliter reactions
Printing chemical libraries on microarrays for fluid phase nanoliter reactions
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DOI：
10.1073/pnas.1530261100
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发表时间：
2003-07-22
影响因子：
11.1
通讯作者：
Diamond, SL
中科院分区：
文献类型：
--
作者：
Gosalia, DN;Diamond, SL
将甘油的单个纳升液滴内的化合物以400 Spot S/CM 2微阵列化到载玻片上。使用气溶胶沉积，随后的试剂和水被计量到每个反应中心，以快速组装不同的多组分反应，而不需要交叉污染或表面连接。这种蛋白质组学技术允许蛋白酶混合物的动力学分析，蛋白酶-底物相互作用，和高通量筛选反应。半胱天冬酶2，4，和6的抑制剂，通过使用352化合物的组合库微阵列在100个载玻片上一式四份，并筛选对半胱天冬酶2，4，和6，以及凝血酶和糜蛋白酶。从一个印刷运行，消耗
Chemical compounds within individual nanoliter droplets of glycerol were microarrayed onto glass slides at 400 SpotS/CM2. Using aerosol deposition, subsequent reagents and water were metered into each reaction center to rapidly assemble diverse multicomponent reactions without crosscontarnination or the need for surface linkage. This proteomics technique allowed the kinetic profiling of protease mixtures, protease-substrate interactions, and high-throughput screening reactions. An inhibitor of caspases 2, 4, and 6 was identified by using a 352-compound combinatorial library microarrayed in quadruplicates on 100 slides and screened against caspases 2, 4, and 6, as well as thrombin and chymotrypsin. From one printing run that consumes