Identification and characterization of the human SOX6 promoter
Identification and characterization of the human SOX6 promoter
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DOI：
10.1016/j.bbrc.2007.03.133
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发表时间：
2007-06-01
影响因子：
3.1
通讯作者：
Chung, Ung-il
中科院分区：
文献类型：
--
作者：
Ikeda, Toshiyuki;Saito, Taku;Chung, Ung-il
本研究试图确定和表征胚胎启动子Sox 6，软骨分化的决定性调节。初步鉴定了人和小鼠Sox 6的共同转录起始区，其包含高度保守的序列A-box。串联重复的A-box有很强的转录活性，无论是在基础水平和响应Sox 9。携带4xA-box-DsRed 2报告基因的细胞仅在软骨形成分化时发荧光。然后在A-box的3'半中鉴定46-bp核心增强子区域（CES 6），在其中鉴定C/EBP结合基序。过表达的C/EBP β激活了Sox 6启动子，缺乏C/EBP基序的突变型4xCES 6构建体失去了它们的基础活性。CES 6与细胞核提取物形成特异性复合物，该复合物被抗C/EBP β抗体超移位，体外翻译的C/EBP β特异性结合CES 6。因此，我们成功地确定了Sox 6启动子及其核心增强子，并表征了与调控转录因子的相互作用。(c)2007年爱思唯尔公司All rights reserved.
The present study attempted to identify and characterize the embryonic promoter of Sox6, a determinant regulator of chondrogenic differentiation. A common transcription start region for human and mouse Sox6 was initially identified, which contained a highly conserved sequence, A-box. Tandem repeats of A-box had a strong transcriptional activity both at the basal level and in response to Sox9. Cells carrying the 4xA-box-DsRed2 reporter fluoresced only upon chondrogenic differentiation. The 46-bp core enhancer region (CES6) was then identified in the 3' half of A-box, within which a C/EBP-binding motif was identified. Overexpressed C/EBP beta activated the Sox6 promoter, and mutant 4xCES6 constructs lacking the C/EBP motif lost their basal activity. CES6 and nuclear extracts formed a specific complex, which was supershifted by anti-C/EBP beta antibody, and in vitro translated C/EBP beta specifically bound to CES6. Thus, we successfully identified the Sox6 promoter and its core enhancer and characterized the interactions with regulatory transcription factors. (c) 2007 Elsevier Inc. All rights reserved.