Burkholderia latens sp nov., Burkholderia diffusa sp nov., Burkholderia arboris sp nov., Burkholderia seminalis sp nov and Burkholderia metallica sp nov., novel species within the Burkholderia cepacia complex
Burkholderia latens sp nov., Burkholderia diffusa sp nov., Burkholderia arboris sp nov., Burkholderia seminalis sp nov and Burkholderia metallica sp nov., novel species within the Burkholderia cepacia complex
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DOI：
10.1099/ijs.0.65634-0
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发表时间：
2008-07-01
影响因子：
2.8
通讯作者：
Vandamme, Peter
中科院分区：
文献类型：
--
作者：
Vanlaere, Elke;LiPuma, John J.;Vandamme, Peter
采用多相分类学方法对洋葱伯克霍尔德菌复合群（Burkholderia cepacia complex，Bcc）分离株的5个recA基因簇进行了分类学定位。根据16 S rRNA和recA基因序列相似性、多位点序列分型（MLST）数据和中间DNA-DNA结合值，表明这5个基因簇代表了Bcc中的5个新种。这些物种的生化鉴定是困难的，因为大多数BCC物种的情况。然而，这些新物种的鉴定可以通过recA基因序列分析，MLST和BOX-PCR分析和recA RFLP分析来完成。对于诊断实验室，recA基因序列分析提供了准确性和简单性的最佳组合。基于这些结果，我们提出了五个新的BCC种，Burkholderia latens sp. nov.（模式菌株FIRENZE 3（T）=LMG 24064（T）=CCUG 54555（T））、扩散伯克霍尔德氏菌（Burkholderia diffusa sp. nov.）（模式菌株AU 1075（T）=LMG 24065（T）=CCUG 54558（T））、树伯克霍尔德氏菌（Burkholderia arboris sp. nov.）（模式菌株ES 0263 A（T）=LMG 24066（T）=CCUG 54561（T）），精液伯克霍尔德氏菌，新种（类型菌株AU 0475（T）=LMG 24067（T）=CCUG 54564（T））和金属伯克霍尔德氏菌属新种（类型菌株AU 0553（T）=LMG 24068（T）=CCUG 54567（T））。在本研究中，我们还表明，Burkholderia ubonensis应被视为BCC的成员。
The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach, The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA-DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3(T) =LMG 24064(T) =CCUG 54555(T)), Burkholderia diffusa sp. nov. (type strain AU1075(T) =LMG 24065(T) =CCUG 54558(T)), Burkholderia arboris sp. nov. (type strain ES0263A(T) =LMG 24066(T) =CCUG 54561(T)), Burkholderia seminalis sp. nov. (type strain AU0475(T) =LMG 24067(T) =CCUG 54564 (T)) and Burkholderia metallica sp. nov. (type strain AU0553(T) =LMG 24068(T) =CCUG 54567(T)). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc.