THE ELECTROPHYSIOLOGICAL EFFECTS OF ACETYLCHOLINE IN SINGLE HUMAN ATRIAL CELLS
THE ELECTROPHYSIOLOGICAL EFFECTS OF ACETYLCHOLINE IN SINGLE HUMAN ATRIAL CELLS
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DOI：
10.1016/s0022-2828(87)80531-x
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发表时间：
1987-12-01
影响因子：
5
通讯作者：
CARMELIET, E
中科院分区：
文献类型：
--
作者：
HEIDBUCHEL, H;VEREECKE, J;CARMELIET, E
从人心房分离单细胞。用全细胞钳技术测量这些细胞中的离子电流。去极化钳步骤期间的时间依赖性电流可被描述为由于Ca电流和瞬态外向电流。在超极化电压阶跃期间观察到时间依赖性内向电流。这些电流由内向整流器iK1携带，而在某些细胞中也存在起搏电流。乙酰胆碱的作用在-50 mV的负电位下进行了研究。乙酰胆碱诱导内向整流钾电流。乙酰胆碱诱导的电流在动力学和Ba敏感性方面不同于iK1。乙酰胆碱的作用由于脱敏而随时间降低。人心房肌细胞对乙酰胆碱的电反应与豚鼠心房肌细胞对乙酰胆碱的反应性质相似。
Single cells were isolated from human atria. The ionic currents in these cells were measured using the whole cell clamp technique. Time-dependent currents during depolarizing clamp steps can be described as due to Ca current and transient outward currents. Time-dependent inward currents were seen during hyperpolarizing voltage steps. These currents are carried by the inward rectifier iK1, while in some cells also an if pacemaker current is present. The effect of acetylcholine was investigated at potentials negative to -50 mV. Acetylcholine induces an inwardly rectifying K current. The acetylcholine-induced current is different from iK1 in kinetics and Ba-sensitivity. The effect of acetylcholine decreases in time due to desensitization. The electrical response of the human atrial cells to acetylcholine is qualitatively similar to the effect of acetylcholine in guinea-pig atrial cells.