Tracheal defect repair using a PLGA–collagen hybrid scaffold reinforced by a copolymer stent with bFGF-impregnated gelatin hydrogel
Tracheal defect repair using a PLGA–collagen hybrid scaffold reinforced by a copolymer stent with bFGF-impregnated gelatin hydrogel
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DOI：
10.1007/s00383-010-2609-2
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发表时间：
2010-04
影响因子：
1.8
通讯作者：
Yukihiro Tatekawa;N. Kawazoe;Guoping Chen;Y. Shirasaki;H. Komuro;M. Kaneko
中科院分区：
文献类型：
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作者：
Yukihiro Tatekawa;N. Kawazoe;Guoping Chen;Y. Shirasaki;H. Komuro;M. Kaneko
目的应用碱性成纤维细胞生长因子(碱性成纤维细胞生长因子)-明胶水凝胶修复气管缺损，并比较生物材料与天然材料的异同。材料与方法在15只实验兔的颈段气管内建立全层前路缺损模型。用聚乳酸-乙醇酸共聚物(PLGA)编织网状物和胶原海绵复合支架植入缺损区。植入的气管由聚己内酯和聚乳酸粗纤维网组成的共聚物支架加固。明胶水凝胶用于提供碱性成纤维细胞生长因子的缓释。将重建的气管分为3组：无明胶水凝胶(对照组，n=5)、明胶加生理盐水凝胶(明胶组，n=5)、含100μg碱性成纤维细胞生长因子明胶水凝胶(碱性成纤维细胞生长因子组，n=5)。每组各5只，分别于术后1个月、3个月、6个月各处死1只，进行组织学评价。术后6个月对标本进行生物力学性能评价。结果术后6个月内，缺损区的刚性支撑物基本保持。碱性成纤维细胞生长因子组术后3个月在宿主软骨残端之间可见新生软骨，术后6个月可见有限的新生软骨生长和上皮化。结论应用碱性成纤维细胞生长因子可获得较好的机械强度，但软骨生长较差。
PurposeWe studied the regenerated cartilage in tracheal defect repair and compared the bio-materials used versus native trachea using basic fibroblast growth factor (bFGF)-impregnated gelatin hydrogel.Materials and methodsA full-thickness anterior defect was created in the cervical trachea of 15 experimental rabbits. The defect was implanted with a hybrid scaffold of poly(lactic-co-glycolic acid) (PLGA) knitted mesh and collagen sponge. The implanted trachea was reinforced with a copolymer stent of polycaprolactone and poly(lactic acid) coarse fiber mesh. A gelatin hydrogel was used for providing a sustained release of bFGF. The reconstructed tracheas were divided into three groups with wrapped materials; without gelatin hydrogel (control group,n= 5), a gelatin hydrogel with saline (gelatin group,n= 5), and a gelatin hydrogel with 100 μg of bFGF (bFGF group,n= 5). One of the five rabbits in each group at 1 month after operation, one at 3 months, and three at 6 months were killed and the engineered tracheas were evaluated histologically. Biomechanical properties were evaluated on samples at 6 months postoperatively.ResultsThe rigid support in the defect portion was maintained during 6 months postoperatively. The newly regenerated cartilages were recognized between the host cartilage stumps at 3 months postoperatively in the bFGF group, and limited new cartilage growth and epithelialization were observed at 6 months postoperatively.ConclusionsThe experiment shows that using bFGF, better mechanical strength was obtained but with poor cartilage growth.