Angiotensin II and VEGF are involved in angiogenesis induced by short-term exercise training
Angiotensin II and VEGF are involved in angiogenesis induced by short-term exercise training
复制标题
DOI：
10.1152/ajpheart.2001.281.3.h1163
复制
发表时间：
2001-09-01
影响因子：
4.8
通讯作者：
Greene, AS
中科院分区：
文献类型：
--
作者：
Amaral, SL;Papanek, PE;Greene, AS
我们实验室的结果表明，在电刺激诱导的血管生成中，有一条涉及血管紧张素II I型（AT(1)）受体和血管内皮生长因子（VEGF）的途径。本研究调查了短期运动训练诱导血管生成的类似机制。在训练前7天和整个训练期间，雄性spraguale - dawley大鼠在其饮用水中分别服用卡托普利或氯沙坦。大鼠进行了为期3天的跑步机训练。在麻醉下切除胫骨前肌和腓肠肌，用福尔马林（血管密度）或液氮冷冻（VEGF表达）进行轻度固定。在对照组中，跑步机训练导致所有肌肉血管密度显著增加。然而，氯沙坦或卡托普利完全阻断了运动诱导的血管生成。Western blot分析显示，运动对照组VEGF表达升高，氯沙坦和卡托普利均可阻断这种升高。使用VEGF中和抗体直接证实了VEGF的作用。这些结果证实了血管紧张素II和VEGF在运动诱导的血管生成中的作用。
Results from our laboratory have suggested a pathway involving angiotensin II type I (AT(1)) receptors and vascular endothelial growth factor (VEGF) in angiogenesis induced by electrical stimulation. The present study investigated if similar mechanisms underlie the angiogenesis induced by short-term exercise training. Seven days before training and throughout the training period, male Sprague-Dawley rats received either captopril or losartan in their drinking water. Rats underwent a 3-day treadmill training protocol. The tibialis anterior and gastrocnemius muscles were harvested under anesthesia and lightly fixed in formalin (vessel density) or frozen in liquid nitrogen (VEGF expression). In controls, treadmill training resulted in a significant increase in vessel density in all muscles studied. However, the angiogenesis induced by exercise was completely blocked by either losartan or captopril. Western blot analysis showed that VEGF expression was increased in the exercised control group, and both losartan and captopril blocked this increase. The role of VEGF was directly confirmed using a VEGF-neutralizing antibody. These results confirm the role of angiotensin II and VEGF in angiogenesis induced by exercise.