LOCALIZATION OF GLYCINE RECEPTORS IN THE RAT CENTRAL NERVOUS-SYSTEM - AN IMMUNOCYTOCHEMICAL ANALYSIS USING MONOCLONAL-ANTIBODY
LOCALIZATION OF GLYCINE RECEPTORS IN THE RAT CENTRAL NERVOUS-SYSTEM - AN IMMUNOCYTOCHEMICAL ANALYSIS USING MONOCLONAL-ANTIBODY
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DOI：
10.1016/0306-4522(88)90263-1
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发表时间：
1988-05-01
期刊：
影响因子：
3.3
通讯作者：
TOHYAMA, M
中科院分区：
文献类型：
--
作者：
ARAKI, T;YAMANO, M;TOHYAMA, M
用抗亲和纯化的甘氨酸受体的单抗对大鼠脑内甘氨酸受体的定位进行了免疫细胞化学检测。甘氨酸受体主要集中在脑干下部，而间脑和前脑除少数间脑核团外，其余部位均未见免疫反应。在脑运动核团、网状结构、臂旁区、耳蜗背、腹侧核、背、腹侧被盖核团中受体密度最高。免疫反应阳性成分在不同脑区的分布存在差异。在小脑皮质，免疫反应仅见于浦肯野细胞的树突。在耳蜗核、三叉神经运动核、臂旁区、外侧网状核、外侧丘脑背核、小脑核、三叉神经脊束核、前角和网状结构的细胞膜上可见甘氨酸受体的表达。在其他区域，受体均匀分布在神经纤维上。
The localization of glycine receptors was immunocytochemically examined in the rat brain using a monoclonal antibody against the affinity-purified glycine receptor. Glycine receptor were concentrated in the lower brainstem, whereas no immunoreactivity was observed in the diencephalon and forebrain except in a few diencephalic nuclei. The highest density of receptors was found in the cranial motor nuclei, reticular formation, parabrachial area, dorsal and ventral cochlear nucelei, and dorsal and ventral tegmental nuclei. Differences were observed in the distribution of immunoreactive elements in the various brain regions. In the cerebellar cortex, the immunoreactivity was exclusively seen along the dendrites of the Purkinje cells. On the other hand, glycine receptors were detected on the cellular membrane of the soma of the cochlear nuclei, trigeminal motor nucleus, parabrachial area, lateral reticular nucleus, dorsal nucleus of the lateral lemniscus, cerebellar nuclei, trigeminal spinal nucleus, anterior horn and reticular formation. In other regions, the receptors were evenly distributed throughout the neuropil.