The role of multiple SOS boxes upstream of the Mycobacterium tuberculosis lexA gene -: identification of a novel DNA-damage-inducible gene
The role of multiple SOS boxes upstream of the Mycobacterium tuberculosis lexA gene -: identification of a novel DNA-damage-inducible gene
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DOI：
10.1099/00221287-148-11-3609
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发表时间：
2002-11-01
期刊：
影响因子：
2.8
通讯作者：
Davis, EO
中科院分区：
文献类型：
--
作者：
Dullaghan, EM;Brooks, PC;Davis, EO
在结核分枝杆菌莱克萨基因的上游发现了4个潜在的莱克萨结合位点。对lexA-lacZ报告基因构建体中这些位点的突变分析表明，DNA损伤介导的莱克萨酶表达调控仅需要这些SOS盒中的一个。一个新的DNA损伤诱导基因，Rv 2719 c，被确定为相对于莱克萨分歧转录;其他三个SOS盒被发现参与调节这种新的分枝杆菌特异性基因的表达。SOS盒位于它们所调控的基因的相应启动子区域。
Four potential binding sites for LexA were identified upstream of the Mycobacterium tuberculosis lexA gene. A mutational analysis of these sites in a lexA-lacZ reporter construct revealed that only one of these SOS boxes was required for DNA-damage-mediated regulation of lexA expression. A novel DNA-damage-inducible gene, Rv2719c, was identified that was divergently transcribed relative to lexA; the other three SOS boxes were found to be involved in regulating expression of this novel mycobacterial-specific gene. The SOS boxes lay in the respective promoter regions of the genes that they regulated.