Effects of saturated palmitic acid and omega-3 polyunsaturated fatty acids on Sertoli cell apoptosis
Effects of saturated palmitic acid and omega-3 polyunsaturated fatty acids on Sertoli cell apoptosis
复制标题
DOI：
10.1080/19396368.2018.1471554
复制
发表时间：
2018-01-01
影响因子：
2.4
通讯作者：
Yao, Bing
中科院分区：
文献类型：
--
作者：
Hu, Xuechun;Ge, Xie;Yao, Bing
肥胖被认为会对男性精液质量产生负面影响，并伴有血浆中游离脂肪酸（FFA）代谢失调。然而，FFA 失调对精液质量的影响以及支持细胞的参与仍不清楚。在本研究中，我们报告肥胖通过失调的 FFA 降低了支持细胞的活力。我们观察到，在接受高脂饮食 (HFD) 的肥胖小鼠睾丸中，支持细胞凋亡率增加，同时 FFA 水平升高。此外，活性氧的水平升高。此外，我们通过体外测定证明，饱和棕榈酸 (PA)（血浆中最常见的饱和 FFA）会以时间和剂量依赖性方式导致 TM4 Sertoli 细胞的细胞活力下降。在原代小鼠支持细胞中也发现了类似的发现。与饱和 FFA 相比，omega-3 (-3) 多不饱和脂肪酸 (PUFA) 可在生理相关水平上保护支持细胞免受 PA 诱导的脂毒性。这些结果表明，饱和脂肪酸的脂毒性可能是肥胖引起的支持细胞凋亡的原因，从而导致精液质量下降。另外，-3 PUFA可被归类为保护性FFA。缩写：FFA：游离脂肪酸； HFD：高脂肪饮食； SD：标准饮食； PA：棕榈酸； PUFA：多不饱和脂肪酸； AI：细胞凋亡指数； MTT：3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四唑溴化物； ROS：活性氧； HE：苏木精和伊红； WT1：肾母细胞瘤1； NAFLD：非酒精性脂肪肝病； DCFH-DA：2'，7'二氯荧光素二乙酸酯； 36B4：酸性核糖体磷蛋白P0； SD：标准差； EPA：二十碳五烯酸； PI：碘化丙啶； DHA：二十二碳六烯酸。
Obesity is believed to negatively affect male semen quality and is accompanied by dysregulation of free fatty acid (FFA) metabolism in plasma. However, the implication of dysregulated FFA on semen quality and the involvement of Sertoli cells remain unclear. In the present study, we report obesity decreased Sertoli cell viability through dysregulated FFAs. We observed an increased rate of apoptosis in Sertoli cells, accompanied with elevated FFA levels, in the testes of obese mice that were provided a high-fat diet (HFD). Moreover, the levels of reactive oxygen species were elevated. Furthermore, we demonstrated by in vitro assays that saturated palmitic acid (PA), which is the most common saturated FFA in plasma, led to decreased cell viability of TM4 Sertoli cells in a time- and dose-dependent manner. A similar finding was noted in primary mouse Sertoli cells. In contrast to saturated FFA, omega-3 (-3) polyunsaturated fatty acids (PUFAs) protected Sertoli cells from PA-induced lipotoxicity at the physiologically relevant levels. These results indicated that the lipotoxicity of saturated fatty acids might be the cause of obesity-induced Sertoli cell apoptosis, which leads to decreased semen quality. In addition, -3 PUFAs could be classified as protective FFAs.Abbreviations: FFA: free fatty acid; HFD: high-fat diet; SD: standard diet; PA: palmitic acid; PUFA: polyunsaturated fatty acid; AI: apoptotic index; MTT: 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide; ROS: reactive oxygen species; HE: Hematoxylin and eosin; WT1: Wilm Tumor 1; NAFLD: non- alcoholic fatty liver disease; DCFH-DA: 2', 7' dichloroFLuorescin diacetate; 36B4: acidic ribosomal phosphoprotein P0; SD: standard deviation; EPA: eicosapentaenoic acid; PI: propidium iodide; DHA: docosahexenoic acid.