Interstitial telomere-like repeats in the monocot family Araceae
Interstitial telomere-like repeats in the monocot family Araceae
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DOI：
10.1111/boj.12231
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发表时间：
2015
影响因子：
2.4
通讯作者：
Aretuza Sousa;S. Renner
中科院分区：
文献类型：
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作者：
Aretuza Sousa;S. Renner
将分子细胞遗传学和染色体数量变化的系统发育模型结合起来，可以揭示进化变化的类型，从而解释今天观察到的单倍体数量。应用于染色体数目为 2n = 8 至 2n = 160 的单子叶植物科天南星科，这种方法表明，下降的异倍体在当前染色体数目的进化中比多倍体发挥了更大的作用。为了测试这一点，我们使用端粒重复探针、5S rDNA 和 45S rDNA 以及覆盖该科 118 个属（其中许多属多个属）的质体系统发育树，对 11 个属的 14 个物种进行了分子细胞遗传学分析。我们获得了 6 个物种的新染色体计数，利用该科的所有可用计数对染色体数目进化进行了建模，并使用三种探针（5S rDNA、45S rDNA 和拟南芥样端粒）对 14 个物种（2n = 14 至 2n = 60）进行了荧光原位杂交。祖先状态重建为天南星科中下降的二倍体和间质端粒的重要作用提供了支持。在红掌、温德林吉和白鹤芋中检测到重复序列（ITR），均为 2n = 30。红掌中的 ITR 信号数量（最多 12 个）是迄今为止在被子植物中报道的最高的，而位于温德林吉的中心粒周围区域的大重复序列是以前仅在裸子植物苏铁和松属中报道的类型。 © 2014 伦敦林奈学会，《林奈学会植物学杂志》，2015 年，177，15–26。
Combining molecular cytogenetics and phylogenetic modelling of chromosome number change can shed light on the types of evolutionary changes that may explain the haploid numbers observed today. Applied to the monocot family Araceae, with chromosome numbers of 2n = 8 to 2n = 160, this type of approach has suggested that descending dysploidy has played a larger role than polyploidy in the evolution of the current chromosome numbers. To test this, we carried out molecular cytogenetic analyses in 14 species from 11 genera, using probes for telomere repeats, 5S rDNA and 45S rDNA and a plastid phylogenetic tree covering the 118 genera of the family, many with multiple species. We obtained new chromosome counts for six species, modelled chromosome number evolution using all available counts for the family and carried out fluorescence in situ hybridization with three probes (5S rDNA, 45S rDNA and Arabidopsis-like telomeres) on 14 species with 2n = 14 to 2n = 60. The ancestral state reconstruction provides support for a large role of descending dysploidy in Araceae, and interstitial telomere repeats (ITRs) were detected in Anthurium leuconerum, A. wendlingeri and Spathyphyllum tenerum, all with 2n = 30. The number of ITR signals in Anthurium (up to 12) is the highest so far reported in angiosperms, and the large repeats located in the pericentromeric regions of A. wendlingeri are of a type previously reported only from the gymnosperms Cycas and Pinus. © 2014 The Linnean Society of London, Botanical Journal of the Linnean Society, 2015, 177, 15–26.