An ultrasensitive fluorescent assay for the in vivo quantification of superoxide radical in organisms
An ultrasensitive fluorescent assay for the in vivo quantification of superoxide radical in organisms
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DOI：
10.1016/j.ab.2005.09.013
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发表时间：
2005-12-01
影响因子：
2.9
通讯作者：
Sideris, T
中科院分区：
文献类型：
--
作者：
Georgiou, CD;Papapostolou, I;Sideris, T
超氧阴离子自由基是参与多种生物现象的氧化应激的重要参数，因此，对其进行体内研究具有十分重要的意义。然而，由于其寿命短，生理浓度很低，其准确检测是一个挑战。目前的所有检测都是定性的和非特异性的，充其量都是在体外进行的。目前的基于二氢乙啶的检测克服了所有这些问题，并引入了以下新奇之处。首先，它测量动物、植物和微生物体内超氧化物的产生。其次，它是超灵敏的，非常简单，可以测量生物样品中低至1.5pmoL的超氧阴离子自由基，低至5毫克。第三，该分析的高灵敏度首次使在生理和模拟的非生理条件下测量超氧阴离子自由基的实际形成速度成为可能。(C)2005 Elsevier Inc.保留所有权利。
Superoxide radical is a very important parameter of oxidative stress involved in a variety of biological phenomena; therefore, its in vivo study is of utmost significance. However, its accurate detection is a challenge due to its short lifetime and its very low physiological concentration. All current assays are qualitative and nonspecific, and at best they are performed in vitro. The current dihydroethidineb-ased assay overcomes all these problems and introduces the following novelties. First, it measures the in vivo superoxide production in animals, plants, and microorganisms. Second, it is ultrasensitive and very simple in that it can measure superoxide radical as low as 1.5 pmol in biological samples as low as 5 mg. Third, the very high sensitivity of the assay renders possible, for the first time, the measurement of the actual rate of formation of superoxide radical under physiological and simulated nonphysiological conditions. (c) 2005 Elsevier Inc. All rights reserved.