AN EXTRACELLULAR MICROELECTRODE ARRAY FOR MONITORING ELECTROGENIC CELLS IN CULTURE
AN EXTRACELLULAR MICROELECTRODE ARRAY FOR MONITORING ELECTROGENIC CELLS IN CULTURE
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DOI：
10.1016/0956-5663(90)80011-2
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发表时间：
1990-01-01
影响因子：
12.6
通讯作者：
WILKINSON, CDW
中科院分区：
文献类型：
--
作者：
CONNOLLY, P;CLARK, P;WILKINSON, CDW
本文介绍了一种平面阵列微电极监测培养细胞的电活动。该装置允许在电极上方的绝缘层中结合表面形貌特征。半导体技术用于金电极的制造以及氮化硅绝缘层的沉积和图案化。已经使用鸡胚肌细胞的心脏细胞培养物测试了电极，并且所培养的细胞的物理跳动与获得的同时的细胞外电压测量相关。发现细胞的细胞外刺激可以通过用于记录的相同电极进行。
This paper describes a planar array of microelectrodes developed for monitoring the electrical activity of cells in culture. The device allows the incorporation of surface topographical features in an insulating layer above the electrodes. Semiconductor technology is employed for the fabrication of the gold electrodes and for the deposition and patterning of an insulating layer of silicon nitride. The electrodes have been tested using a cardiac cell culture of chick embryo myocytes, and the physical beating of the cultured cells correlated with the simulataneous extracellular voltage measurements obtained. It was found that extracellular stimulation of the cells was possible via the same electrodes used for recording.