The tobacco gene Ntcyc07 confers arsenite tolerance in Saccharomyces cerevisiae by reducing the steady state levels of intracellular arsenic
The tobacco gene Ntcyc07 confers arsenite tolerance in Saccharomyces cerevisiae by reducing the steady state levels of intracellular arsenic
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DOI：
10.1016/j.febslet.2008.02.030
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发表时间：
2008-03-19
期刊：
影响因子：
3.5
通讯作者：
Hwang, Seongbin
中科院分区：
文献类型：
--
作者：
Mok, Young Geun;Lee, Byoung Doo;Hwang, Seongbin
我们克隆了一个植物基因，Ntcyc07，赋予砷耐受性，通过表达烟草表达文库在野生型酵母（Y800）。Ntcyc07的表达增加了对As（III）的耐受性并降低了其积累，这表明增强的As（III）耐受性是由于细胞内砷水平的降低。有趣的是，Ntcyc07的表达增加了As（III）输出载体ACR 3的表达，但抑制了As（III）摄取通道FPS 1的表达。Ntcyc07p与ACR3的转录激活因子Acr1p相互作用，但不与ACR3启动子相互作用。综上所述，数据表明Ntcyc07p通过增加ACR 3的表达和减少FPS 1的表达来降低细胞内As（III）水平，从而促进As（III）耐受性。(C)2008年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
We cloned a plant gene, Ntcyc07, conferring arsenite tolerance by expressing a tobacco expression library in WT yeast (Y800). Expression of Ntcyc07 increased the tolerance to As(III) and decreased its accumulation, suggesting that the enhanced As(III) tolerance resulted from a reduction of the intracellular arsenic level. Interestingly, expression of Ntcyc07 increased the expression of the As(III) export carrier ACR3, but repressed that of As(III) uptake channel FPS1. Ntcyc07p interacted with Acr1p, which is the transcriptional activator of ACR3, but not with the ACR3 promoter. Taken together, the data indicated that Ntcyc07p promoted As(HI) tolerance by decreasing the intracellular level of As(III) via increasing the expression of ACR3 and reducing that of FPS1. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.