Supt20 is required for development of the axial skeleton.
Supt20 is required for development of the axial skeleton.
复制标题
Supt20 是开发轴向骨架所必需的。
DOI：
10.1016/j.ydbio.2016.11.009
复制
发表时间：
2017
影响因子：
2.7
通讯作者：
Zohn,IreneE
中科院分区：
文献类型：
--
作者：
Warrier,Sunita;Nuwayhid,Samer;Sabatino,JuliaA;Sugrue,KelseyF;Zohn,IreneE
Somitogenesis and subsequent axial skeletal development is regulated by the interaction of pathways that determine the periodicity of somite formation, rostrocaudal somite polarity and segment identity. Here we use a hypomorphic mutant mouse line to demonstrate thatSupt20(Suppressor of Ty20) is required for development of the axial skeleton.Supt20hypomorphs display fusions of the ribs and vertebrae at lower thoracic levels along with anterior homeotic transformation of L1 to T14. These defects are preceded by reduction of the rostral somite and posterior shifts inHoxgene expression. While cycling of Notch target genes in the posterior presomitic mesoderm (PSM) appeared normal, expression ofLfngwas reduced. In the anterior PSM,Mesp2expression levels and cycling were unaffected; yet, expression of downstream targets such asLfng, Ripply2,Mesp1andDll3in the prospective rostral somite was reduced accompanied by expansion of caudal somite markers such asEphrinB2andHes7. Supt20interacts with the Gcn5-containing SAGA histone acetylation complex.Gcn5hypomorphic mutant embryos show similar defects in axial skeletal development preceded by posterior shift ofHoxc8andHoxc9gene expression. We demonstrate thatGcn5andSupt20hypomorphs show similar defects in rostral-caudal somite patterning potentially suggesting shared mechanisms.
