Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion
Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion
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DOI：
10.1016/j.micinf.2009.03.007
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发表时间：
2009-04-01
影响因子：
5.8
通讯作者：
Mortara, Renato A.
中科院分区：
文献类型：
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作者：
da Silva, Claudio V.;Kawashita, Silvia Y.;Mortara, Renato A.
筛选克氏锥虫基因组数据库中的假设蛋白质，这些蛋白质显示出被分泌或膜锚定的高概率，因此可能参与宿主细胞入侵。选择编码显示高分泌概率的21 kDa蛋白质的序列。克隆该蛋白序列后，结果表明它是一种普遍存在的蛋白，由胞外无鞭毛体分泌。重组体（P21-His（6））以剂量依赖性方式粘附于HeLa细胞。用P21-His（6）预处理宿主细胞可抑制G株和CL株胞外无鞭毛体的细胞侵袭。另一方面，当蛋白质与无鞭毛体同时加入宿主细胞时，观察到细胞侵袭增加。用P21-His（6）预处理宿主细胞增强了亚周期锥鞭毛体的侵袭。抗P21多克隆抗体仅能抑制G株无鞭毛体和后环锥鞭毛体的侵袭。提示P21可能参与了T. cruzi细胞浸润。我们假设P21可以分泌在并置寄生虫-宿主细胞中，并触发导致寄生虫内化的尚不清楚的信号事件。(C)2009年Elsevier Masson SAS。All rights reserved.
Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence that codes for a 21 kDa protein that showed high probability of being secreted was selected. After cloning this protein sequence, the results showed that it was a ubiquitous protein and secreted by extracellular amastigotes. The recombinant form (P21-His(6)) adhered to HeLa cells in a dose-dependent manner. Pretreatment of host cells with P21-His(6) inhibited cell invasion by extracellular amastigotes from G and CL strains. On the other hand, when the protein was added to host cells at the same time as amastigotes, an increase in cell invasion was observed. Host-cell pretreatment with P21-His(6) augmented invasion by metacyclic trypomastigotes. Moreover, polyclonal antibody anti-P21 inhibited invasion only by extracellular amastigotes and metacyclic trypomastigotes from G strain. These results suggested that P21 might be involved in T. cruzi cell invasion. We hypothesize that P21 could be secreted in the juxtaposition parasite-host cell and triggers signaling events yet unknown that lead to parasite internalization. (C) 2009 Elsevier Masson SAS. All rights reserved.