Secretion of the glucose-regulated selenoprotein SEPS1 from hepatoma cells
Secretion of the glucose-regulated selenoprotein SEPS1 from hepatoma cells
复制标题
DOI：
10.1016/j.bbrc.2007.03.018
复制
发表时间：
2007-05-11
影响因子：
3.1
通讯作者：
Collier, Greg R.
中科院分区：
文献类型：
--
作者：
Gao, Yuan;Pagnon, Joanne;Collier, Greg R.
SEPS1（也称为硒蛋白 S、SeIS、Tanis 或 VIMP）是一种硒蛋白，主要定位于 ER 膜以及细胞表面。在本报告中，我们证明 SEPS1 蛋白也从肝癌细胞中分泌，但不从所检查的其他五种类型的细胞中分泌。 ER-高尔基体转运抑制剂 Brefeldin A 和蛋白质合成抑制剂放线菌酮可消除这种分泌。使用夹心 ELISA，在 209 名人类受试者中的 65 名受试者的血清中检测到了 SEPS1（31.1%，平均值 = 15.7 +/- 1.1 ng/mL）。人血清的分离表明 SEPS I 与 LDL 相关，并且可能与 VLDL 相关。血浆SEPS I的功能尚不清楚，但可能与脂蛋白代谢有关。 (c) 2007 Elsevier Inc. 保留所有权利。
SEPS1 (also called selenoprotein S, SeIS, Tanis or VIMP) is a selenoprotein, localized predominantly in the ER membrane and also on the cell surface. In this report, we demonstrate that SEPS1 protein is also secreted from hepatoma cells but not from five other types of cells examined. The secretion can be abolished by the ER-Golgi transport inhibitor Brefeldin A and by the protein synthesis inhibitor cycloheximide. Using a sandwich ELISA, SEPS1 was detected in the sera of 65 out of 209 human subjects (31.1%, average = 15.7 +/- 1.1 ng/mL). Fractionation of human serum indicated that SEPS I was associated with LDL and possibly with VLDL. The function of plasma SEPS I is unclear but may be related to lipoprotein metabolism. (c) 2007 Elsevier Inc. All rights reserved.