Hydrodynamic gating for sample introduction on a microfluidic chip
Hydrodynamic gating for sample introduction on a microfluidic chip
复制标题
用于微流控芯片上样品引入的流体动力门控
DOI：
10.1039/b925096d
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Liu, Bi-Feng
中科院分区：
文献类型：
--
作者：
Chen, Pu;Feng, Xiaojun;Liu, Bi-Feng
提出了一种基于流体动力学门控进样的微流控进样方法。它具有简单灵活的流动控制的优点，类似于其模拟物，电动门控注射，但具有强大的驱动力和良好的生物相容性的独特性能，非常适合涉及活生物样品的应用。提出了流体动力学浇注理论，并通过数值模拟和流动显示实验进行了验证。用荧光素进行的研究表明，可以以高重复性（RSD < 1.9%）进样皮升样品。GFP转染的线虫卵的选择性注射被证明具有> 95%的存活率。我们期望所开发的方法对于微流体细胞和生物体分析是潜在有用的，无论是作为样品引入模块还是作为独立的分析仪。
We present a microfluidic sample introduction approach based on a novel flow control mechanism, hydrodynamic gated injection. It has the advantages of easy and flexible flow control, similar to its analog, electrokinetic gated injection, but exhibits the unique properties of strong driving force and good biocompatibility, ideal for applications involving live biological samples. Theories for hydrodynamic gating were proposed and validated by both numerical simulations and flow visualization experiments. An investigation with fluorescein revealed that pico-liter samples can be injected with high repeatability (RSD < 1.9%). Selective injections of GFP-transfected nematode eggs were demonstrated with a survival rate of > 95%. We expect the developed method to be potentially useful for microfluidic cell and organism analysis, either as a sample introduction module or a stand-alone analyzer.