Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1
Localization of iron in Arabidopsis seed requires the vacuolar membrane transporter VIT1
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DOI：
10.1126/science.1132563
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发表时间：
2006-11-24
期刊：
影响因子：
56.9
通讯作者：
Guerinot, Mary Lou
中科院分区：
文献类型：
--
作者：
Kim, Sun A.;Punshon, Tracy;Guerinot, Mary Lou
在以植物性饮食为主的地方，缺铁是一个主要的人类营养问题。利用同步辐射X射线荧光显微断层扫描技术直接观察拟南芥种子中的铁，我们发现铁主要定位在胚胎的原维管束中。当液泡铁吸收转运蛋白VIT1被破坏时，这种定位完全消失。液泡铁储存对幼苗发育也至关重要，因为在铁有限的情况下，vit1 - 1幼苗生长不良。我们揭示了种子生物学的一个基本方面，这最终将有助于培育营养丰富的种子，对人类健康和农业生产力都有益。
Iron deficiency is a major human nutritional problem wherever plant-based diets are common. Using synchrotron x-ray fluorescence microtomography to directly visualize iron in Arabidopsis seeds, we show that iron is localized primarily to the provascular strands of the embryo. This localization is completely abolished when the vacuolar iron uptake transporter VIT1 is disrupted. Vacuolar iron storage is also critical for seedling development because vit1-1 seedlings grow poorly when iron is limiting. We have uncovered a fundamental aspect of seed biology that will ultimately aid the development of nutrient-rich seed, benefiting both human health and agricultural productivity.