Reactive oxygen species induce cyanide-resistant respiration in potato infected by Erwinia carotovora subsp. Carotovora
Reactive oxygen species induce cyanide-resistant respiration in potato infected by Erwinia carotovora subsp. Carotovora
复制标题
活性氧诱导受胡萝卜软腐欧文氏菌亚种感染的马铃薯的抗氰化物呼吸。
DOI：
10.1016/j.jplph.2020.153132
复制
发表时间：
2020-03-01
影响因子：
4.3
通讯作者：
Li, Hongyu
中科院分区：
文献类型：
--
作者：
Hua, Dong;Duan, Jiangong;Li, Hongyu
研究表明，病原菌感染诱导植物体内活性氧（ROS）的过量产生。抗氰呼吸是植物的一种能量耗散途径，也可由病原菌感染诱导。然而，目前还不清楚病原菌感染下的抗氰呼吸的诱导是否是由活性氧引起的。本研究以两个致病性欧文氏菌菌株感染马铃薯块茎，测定了其膜脂过氧化水平和抗氰呼吸能力。此外，分析了马铃薯块茎中StAOX的表达和ROS的调控。此外，在调节抗氰呼吸的Ca 2+途径的作用进行了测定。结果表明，活性氧诱导感染欧文氏菌的马铃薯块茎抗氰呼吸。抗氰呼吸抑制H2 O2的产生。胞内Ca ~（2+）调节马铃薯钙依赖蛋白激酶（StCDPK 1、StCDPK 4和StCDPK 5）的表达，从而间接控制胞内ROS水平。这些结果表明，Ca 2+代谢参与ROS诱导的抗氰呼吸。
Studies have shown that pathogenic bacteria infections induce the overproduction of reactive oxygen species (ROS) in plants. Cyanide-resistant respiration, an energy-dissipating pathway in plants, has also been induced by a pathogenic bacteria infection. However, it is unknown whether the induction of cyanide-resistant respiration under the pathogenic bacteria infection was caused by ROS. In this study, two pathogenic Erwinia strains were used to infect potato tuber, and membrane lipid peroxidation levels and the cyanide-resistant respiration capacity were determined. In addition, StAOX expression and regulation by ROS in potato tuber were analyzed. Moreover, the role of the Ca2+ pathway in regulating cyanide-resistant respiration was determined. The results showed that ROS induced cyanide-resistant respiration in potato tuber infected by Erwinia. Cyanide-resistant respiration inhibited the production of H2O2. Intracellular Ca2+ regulated the expression of calcium-dependent protein kinase (StCDPK1, StCDPK4, and StCDPK5) in potato, which indirectly controlled intracellular ROS levels. These results indicate that Ca2+ metabolism is involved in ROS-induced cyanide-resistant respiration.