Frequency of Candida spp. in the Oral Cavity of Brazilian HIV-Positive Patients and Correlation with CD4 Cell Counts and Viral Load
Frequency of Candida spp. in the Oral Cavity of Brazilian HIV-Positive Patients and Correlation with CD4 Cell Counts and Viral Load
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DOI：
10.1007/s11046-008-9153-9
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发表时间：
2009-02-01
期刊：
影响因子：
5.5
通讯作者：
Koga-Ito, Cristiane Yumi
中科院分区：
文献类型：
--
作者：
Back-Brito, G. N.;Mota, A. J.;Koga-Ito, Cristiane Yumi
本研究的目的是评估念珠菌属的患病率，尤其是C. dubliniensis，在巴西HIV阳性患者的口腔分离株中，将这些结果与CD 4细胞计数和病毒载量相关联。45名经ELISA和Western印迹法诊断为HIV阳性、接受抗逆转录病毒治疗至少1年且无口腔念珠菌病信号的个体（23名女性和22名男性）纳入研究。对照组由45名健康个体组成，在年龄、性别和口腔条件方面与试验组相匹配。收集口腔冲洗液，并通过表型试验进行鉴定。C.使用经验证的多重PCR测定分析分离株中的都柏林氏杆菌。念珠菌在HIV阳性患者的口腔中检测到的数量显著高于对照组（P = 0.0008）。C.在两组中，白色念珠菌是最常见的分离菌种。在HIV组中，C.光叶C. lipolytica，C. krusei，克鲁塞伊C. guilliermondii和C.还发现了近平滑。在对照组中，我们还鉴定了C。和C.都柏林的。从对照个体分离的2株（1.9%，2/108）被鉴定为C. dubliniensis，该物种在HIV组中未得到验证。念珠菌在低病毒载量（< 400拷贝/mmA）患者的口腔中，病毒计数在统计学上较低（P = 0.0230）（3）。念珠菌CD 4细胞计数在不同水平组间无统计学差异（P = 0.1068）。
The aim of this study was to evaluate the prevalence of Candida spp., and particularly C. dubliniensis, among oral isolates from Brazilian HIV-positive patients correlating these results with CD4 cell counts and viral load. Forty-five individuals (23 female and 22 male) diagnosed as HIV-positive by ELISA and Western-blot, under anti-retroviral therapy for at least 1 year and without oral candidosis signals were included in the study. The control group was constituted by 45 healthy individuals, matched to the test group in relation to age, gender, and oral conditions. Oral rinses were collected and the identification was performed by phenotypic tests. The existence of C. dubliniensis among the isolates was analyzed using a validated multiplex PCR assay. Candida spp. were detected at significantly higher number in the oral cavity of HIV-positive patients in relation to the controls (P = 0.0008). C. albicans was the most frequently isolated species in both groups. In the HIV group, C. glabrata, C. lipolytica, C. krusei, C. guilliermondii, and C. parapsilosis were also identified. In the control group, we additionally identified C. tropicalis and C. dubliniensis. Two isolates (1.9%, 2/108) from control individuals were identified as C. dubliniensis and this species was not verified in the HIV group. Candida spp. counts were statistically lower (P = 0.0230) in the oral cavity of patients with low viral load (< 400 copies/mmA(3)). Candida spp. counts did not differ statistically among groups with different levels of CD4 cells counts (P = 0.1068).